![SDS-Page: Recombinant Human KRAS Protein [NBP2-22940] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-KRAS-Protein-SDS-Page-NBP2-22940-img0002.jpg "SDS-Page: Recombinant Human KRAS Protein [NBP2-22940] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Concentration | 0.5 mg/ml |
| Description | A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-186 of Human His-tag KRAS, NCBI Accession Number NP_203524.1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTEYKL VVVGAGGVGK SALTIQLIQN HFVDEYDPTI EDSYRKQVVI DGETCLLDIL DTAGQEEYSA MRDQYMRTGE GFLCVFAINN TKSFEDIHHY REQIKRVKDS EDVPMVLVGN KCDLPSRTVD TKQAQDLARS YGIPFIETSA KTRQRVEDAF YTLVREIRQY RLKKISKEEK TPGCVKIKKC |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | KRAS |
| Purity | >90%, by SDS-PAGE |
| Dilutions |
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| Theoretical MW | 23.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Purity | >90%, by SDS-PAGE |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRAS |
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![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - miRNA-768-3p specifically targets K-ras. Effect of miRNA inhibitors that were reduced by co-culture on the level of pan-RAS, phospho-B-Raf and CD44 and pan-Akt by western blotting. Also shown is the effect of different miRNA inhibitors on the level of K-ras in H520 cells by western blot. Cropped blots are shown and were run under the same experimental conditions. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep02392), licensed under a CC-BY license.](https://images.novusbio.com/images/KRAS-Antibody-3B10-2F2-Western-Blot-H00003845-M01-img0011.jpg "Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - miRNA-768-3p specifically targets K-ras. Effect of miRNA inhibitors that were reduced by co-culture on the level of pan-RAS, phospho-B-Raf and CD44 and pan-Akt by western blotting. Also shown is the effect of different miRNA inhibitors on the level of K-ras in H520 cells by western blot. Cropped blots are shown and were run under the same experimental conditions. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep02392), licensed under a CC-BY license.")