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KRAS Antibody (OTI2C1) - Azide and BSA Free

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Western Blot: KRAS Antibody (OTI2C1) - Azide and BSA Free [NBP2-71079] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS.
Western Blot: KRAS Antibody (OTI2C1) - Azide and BSA Free [NBP2-71079] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS.
Western Blot: KRAS Antibody (OTI2C1) - Azide and BSA Free [NBP2-71079] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB
Clone
OTI2C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71079AF350
Alexa Fluor 405
NBP2-71079AF405
Alexa Fluor 488
NBP2-71079AF488
Alexa Fluor 532
NBP2-71079AF532
Alexa Fluor 594
NBP2-71079AF594
Alexa Fluor 647
NBP2-71079AF647
Alexa Fluor 700
NBP2-71079AF700
Alexa Fluor 750
NBP2-71079AF750
Allophycocyanin
NBP2-71079APC
Biotin
NBP2-71079B
CoraFluor 1
NBP2-71079CL1
DyLight 350
NBP2-71079UV
DyLight 405
NBP2-71079V
DyLight 488
NBP2-71079G
DyLight 550
NBP2-71079R
DyLight 594
NBP2-71079DL594
DyLight 650
NBP2-71079C
DyLight 680
NBP2-71079FR
DyLight 755
NBP2-71079IR
FITC
NBP2-71079F
HRP
NBP2-71079H
Janelia Fluor 525
NBP2-71079JF525
Janelia Fluor 669
NBP2-71079JF669
PE
NBP2-71079PE
PerCP
NBP2-71079PCP
Unconjugated
NBP2-45536
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45536
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KRAS Antibody (OTI2C1) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human KRAS (NP_203524) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KRAS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
Theoretical MW
21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for KRAS Antibody (OTI2C1) - Azide and BSA Free

  • cellular c-Ki-ras2 proto-oncogene
  • c-Ki-ras
  • C-K-RAS
  • GTPase KRas
  • KI-RAS
  • Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog
  • K-Ras 2
  • K-ras p21 protein
  • KRAS1
  • K-RAS2A
  • K-RAS2B
  • KRAS2NS3
  • K-RAS4A
  • K-RAS4B
  • NS
  • oncogene KRAS2
  • PR310 c-K-ras oncogene
  • RASK2c-Kirsten-ras protein
  • transforming protein p21
  • v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Background

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRAS