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KRAS Antibody (OTI1D1)

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Western Blot: KRAS Antibody (OTI1D1) [NBP2-45535] - Total cell lysates from human cancer cell lines PC-3, HeLa and HT-29 cells were analyzed by Western blot. PVDF membrane was probed with Human KRAS (OTI1D1) Antibody ...read more
Western Blot: KRAS Antibody (1D1) [NBP2-45535] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
OTI1D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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KRAS Antibody (OTI1D1) Summary

Immunogen
Full length human recombinant protein of human KRAS (NP_203524) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRAS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
Theoretical MW
21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-45535 in the following application:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KRAS Antibody (OTI1D1)

  • cellular c-Ki-ras2 proto-oncogene
  • c-Ki-ras
  • C-K-RAS
  • GTPase KRas
  • KI-RAS
  • Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog
  • K-Ras 2
  • K-ras p21 protein
  • KRAS1
  • K-RAS2A
  • K-RAS2B
  • KRAS2NS3
  • K-RAS4A
  • K-RAS4B
  • NS
  • oncogene KRAS2
  • PR310 c-K-ras oncogene
  • RASK2c-Kirsten-ras protein
  • transforming protein p21
  • v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Background

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KRAS Antibody (NBP2-45535) (0)

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Review for KRAS Antibody (NBP2-45535) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-45535:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot KRAS NBP2-45535
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5
reviewed by:
Verified Customer
WB Human 07/17/2018
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Summary

ApplicationWestern Blot
Sample Tested3 human cancer cell lines
SpeciesHuman
LotW001

Comments

CommentsTotal cell lysates from PC-3,HeLa and HT-29 cells were subjected to western blot. PVDF membrane were probed with 1 mm/ml Human KRAS Antibody (OTI1D1) (NBP2-45535). A specific band was detected for KRAS at approximately 21 kDa. This experiment was conducted under reducing conditions

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Secondary Antibodies

 

Isotype Controls

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Research Areas for KRAS Antibody (NBP2-45535)

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Blogs on KRAS.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily ...  Read full blog post.

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Recent Reviews

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2
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1
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Verified Customer
07/17/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol KRAS