Home » KIR2DL4/CD158d » KIR2DL4/CD158d Lysates » KIR2DL4/CD158d Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

KIR2DL4/CD158d Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: KIR2DL4 Overexpression Lysate (Adult Normal) [NBL1-12302] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIR2DL4.
Western Blot: KIR2DL4 Overexpression Lysate (Adult Normal) [NBL1-12302] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIR2DL4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

KIR2DL4/CD158d Overexpression Lysate Summary

Description

KIR2DL4/CD158d Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC214354

Accession#: NM_002255

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
KIR2DL4

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for KIR2DL4/CD158d Overexpression Lysate

  • 103AS
  • 15.212
  • CD158 antigen-like family member D
  • CD158d antigen
  • CD158d
  • CD158DKIR103ASKiller cell inhibitory receptor 103AS
  • G9P
  • killer cell immunoglobulin-like receptor 2DL4
  • killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
  • killer Ig receptor
  • KIR103
  • KIR-103AS
  • KIR2DL4
  • MHC class I NK cell receptor KIR103AS
  • natural killer cell inhibitory receptor
  • NK cell receptor

Background

The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human NK cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

KIR3DL1 Antibody
(DX9) [Unconjugated]
MAB1225
KIR3DL1 Antibody (DX9) [Unconjugated]
(5 Publications)
Species: Hu
Applications: Block, CyTOF-reported, Flow
GEM Antibody
NBP1-58906
Immunohistochemistry-Paraffin GEM Antibody
Immunohistochemistry GEM Antibody
Western Blot GEM Antibody
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NKG2D/CD314
Antibody (149810) [Un ...
MAB139
Flow Cytometry NKG2D/CD314 Antibody (149810) [Unconjugated]
Flow Cytometry NKG2D/CD314 Antibody (149810) [Unconjugated]
Flow Cytometry NKG2D/CD314 Antibody (149810) [Unconjugated]
(1 Review)
(101 Publications)
Species: Hu
Applications: CostimT, CyTOF-ready, Flow, Neut, WB
NKG2A/CD159a/KLRC1
Antibody (131411) [Un ...
MAB1059
Flow Cytometry NKG2A/CD159a/KLRC1 Antibody (131411) [Unconjugated]
Flow Cytometry NKG2A/CD159a/KLRC1 Antibody (131411) [Unconjugated]
(17 Publications)
Species: Hu
Applications: CyTOF-ready, Flow
CD94 Antibody
(131412) [Unconjugate
MAB1058
CD94 Antibody (131412) [Unconjugated]
(4 Publications)
Species: Hu
Applications: Block, CyTOF-ready, Flow
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
HLA B Antibody
(2G7B10) - BSA Free
NBP2-61871
ELISA HLA B Antibody (2G7B10) - BSA Free
Flow Cytometry HLA B Antibody (2G7B10) - BSA Free
Immunocytochemistry/ Immunofluorescence HLA B Antibody (2G7B10) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
ILT3/CD85k/LILRB4
Antibody (293623) [Un ...
MAB24251
Flow Cytometry ILT3/CD85k/LILRB4 Antibody (293623) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, WB
MHC Class I
Antibody (OX18) - BSA
NB120-6405
Flow Cytometry MHC Class I Antibody (OX18) - BSA Free
Immunocytochemistry/ Immunofluorescence MHC Class I Antibody (OX18) - BSA Free
Immunocytochemistry/ Immunofluorescence MHC Class I Antibody (OX18) - BSA Free
(36 Publications)
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
KIR2DL3/CD158b2
Antibody (180701) [Un ...
MAB2014
Flow Cytometry KIR2DL3/CD158b2 Antibody (180701) [Unconjugated]
(12 Publications)
Species: Hu
Applications: CyTOF-reported, Flow
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(378 Publications)
Species: Hu
Applications: BA
NKp46/NCR1
Antibody [Unconjugate
AF2225
Agonist Activity NKp46/NCR1 Antibody [Unconjugated]
Immunohistochemistry NKp46/NCR1 Antibody [Unconjugated]
Flow Cytometry NKp46/NCR1 Antibody [Unconjugated]
(1 Review)
(65 Publications)
Species: Mu
Applications: AgAct, CyTOF-ready, Flow, IHC, WB
HLA G Antibody
(MEM-G/1) - BSA Free
NB500-302
Immunohistochemistry HLA G Antibody (MEM-G/1) - BSA Free
Immunohistochemistry HLA G Antibody (MEM-G/1) - BSA Free
Immunohistochemistry HLA G Antibody (MEM-G/1) - BSA Free
(7 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
KIR2DL1/KIR2DS5
Antibody (143211) [Un ...
MAB1844
Flow Cytometry KIR2DL1/KIR2DS5 Antibody (143211) [Unconjugated]
(16 Publications)
Species: Hu
Applications: CyTOF-reported, Flow
HLA C Antibody
(DT-9) - BSA Free
NBP2-50419
HLA C Antibody (DT-9) - BSA Free
(7 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IP
CD161 Antibody
(14F1F11) - BSA Free
NBP2-14845
Immunohistochemistry-Paraffin CD161 Antibody (14F1F11) - BSA Free
Immunohistochemistry-Paraffin CD161 Antibody (14F1F11) - BSA Free
Western Blot CD161 Antibody (14F1F11) - BSA Free
(2 Publications)
Species: Hu
Applications: IHC, IHC-P, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(457 Publications)
Species: Hu
Applications: BA
KIR2DL4/CD158d
Overexpression Lysate
NBL1-12302
KIR2DL4/CD158d Overexpression Lysate
Species: Hu
Applications: WB

Publications for KIR2DL4/CD158d Lysate (NBL1-12302) (0)

There are no publications for KIR2DL4/CD158d Lysate (NBL1-12302).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for KIR2DL4/CD158d Lysate (NBL1-12302) (0)

There are no reviews for KIR2DL4/CD158d Lysate (NBL1-12302). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KIR2DL4/CD158d Lysate (NBL1-12302) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional KIR2DL4/CD158d Products

Research Areas for KIR2DL4/CD158d Lysate (NBL1-12302)

Find related products by research area.

Blogs on KIR2DL4/CD158d.

Check out the latest blog posts on KIR2DL4/CD158d.
Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma .
IFN-gamma [Unconjugated]
285-IF-100
As an alternative, please consider our next generation Recombinant Human IL-2 (BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.
IL-2 [Unconjugated]
202-IL-010
Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
NKG2D/CD314 Antibody (149810) [Unconjugated]
MAB139-100

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our KIR2DL4/CD158d Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol KIR2DL4