![Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1161-img0004.jpg "Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, ChIP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-SSQELSGGPGDGP corresponding to C-Terminus according to NP_005753.1. |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TRIM28 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Immunofluorescence: Successful usage on mouse embryo at the late zygote stage, 5ug/ml. Strong expression of the protein seen in the nucleus of HeLa and U2OS cells. ChIP successful usage for K562 cells is reported in scientific literature (PMID: 21170338). WB: Approx. 120 kDa band observed in HepG2 lysates (calculated MW of 88.5 kDa band according to NP_005753.1). The higher observed MW is explained by sumoylated protein. IHC-P: Human breast shows nuclear staining in epithelial duct cells. IHC successful usage on human gastric cancer surgical specimens is reported in scientific literature (PMID: 19898899). Flow Cytometry: Flow cytometric analysis of HeLa cells. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for KAP1 Antibody (NB100-1161)Find related products by research area.
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| Gene Symbol | TRIM28 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: KAP1 Antibody [NB100-1161] - (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-Paraffin-NB100-1161-img0001.jpg "Immunohistochemistry-Paraffin: KAP1 Antibody [NB100-1161] - (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Flow Cytometry: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/KAP1-Antibody-Flow-Cytometry-NB100-1161-img0002.jpg "Flow Cytometry: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1161-img0003.jpg "Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
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