Home » JNK1/JNK2 » JNK1/JNK2 Antibodies » JNK1/JNK2 Antibody (252323) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

JNK1/JNK2 Antibody (252323) [Unconjugated]

 (6 Publications)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human JNK1, JNK2, and JNK3 (1 ng/lane) were included. Specific bands for JNK1 and JNK2 were detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody ...read more
JNK1/JNK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 10 µg/mL for 3 hours at room ...read more
JNK1/JNK2 was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 25 µg/mL for 3 hours at room temperature. Cells ...read more
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. Specific bands were detected for JNK1/JNK2 at approximately 48 and 58 kDa (as indicated) using 10 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
252323
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2076-SP
Novus Biologicals offers 100% Guarantee

JNK1/JNK2 Antibody (252323) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human JNK1 isoform 2
Specificity
Detects human, mouse, and rat p46 and p54 JNK1/JNK2. Does not detect recombinant JNK3.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
MAB2076 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK1/JNK2 Antibody (252323) [Unconjugated]

  • c-Jun N-terminal kinase 1
  • JNK
  • JNK1
  • JNK1/JNK2
  • JNK1A2
  • JNK21B1/2
  • JNK-46
  • JUN N-terminal kinase
  • MAP kinase 8
  • mitogen-activated protein kinase 8
  • PRKM8
  • SAPK1
  • SAPK1C
  • Stress-activated protein kinase 1
  • stress-activated protein kinase 1c

Background

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
p38 alpha Antibody
[Unconjugated]
AF8691
Simple Western p38 alpha Antibody [Unconjugated]
Western Blot p38 alpha Antibody [Unconjugated]
Immunohistochemistry p38 alpha Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(63 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Crk Antibody
(3G11E8) - BSA Free
NBP2-37568
ELISA Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Flow Cytometry GRAP2 Antibody (OTI1G2)
Flow Cytometry GRAP2 Antibody (OTI1G2)
Western Blot GRAP2 Antibody (OTI1G2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AIMP2 Antibody -
BSA Free
NBP1-81575
Immunohistochemistry-Paraffin AIMP2 Antibody - BSA Free
Immunohistochemistry-Paraffin AIMP2 Antibody - BSA Free
Immunohistochemistry-Paraffin AIMP2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AHSA1 Antibody
(1A2-A8) - Azide and ...
H00010598-M01
Western Blot AHSA1 Antibody (1A2-A8) - Azide and BSA Free
Sandwich ELISA AHSA1 Antibody (1A2-A8) - Azide and BSA Free
(6 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
POLDIP2 Antibody
NBP1-68874
Western Blot POLDIP2 Antibody
Western Blot POLDIP2 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(824 Publications)
Species: Hu
Applications: BA
JNK2 Antibody
(252320) [Unconjugate
MAB1846
Western Blot JNK2 Antibody (252320) [Unconjugated]
Knockout Validated JNK2 Antibody (252320) [Unconjugated]
Immunocytochemistry JNK2 Antibody (252320) [Unconjugated]
(2 Reviews)
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, WB
MKK4/MEK4 Antibody
(OTI8A8)
NBP1-47839
Flow Cytometry MKK4/MEK4 Antibody (OTI8A8)
Immunocytochemistry/ Immunofluorescence MKK4/MEK4 Antibody (OTI8A8)
Immunocytochemistry/ Immunofluorescence MKK4/MEK4 Antibody (OTI8A8)
(1 Review)
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
c-Fos Antibody
(2H2) - BSA Free
NBP2-50037
Western Blot c-Fos Antibody (2H2) - BSA Free
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2) - BSA Free
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2) - BSA Free
(1 Review)
(24 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(51 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, Simple Western, WB
SGSM3 Antibody -
BSA Free
NBP2-13304
Immunohistochemistry-Paraffin SGSM3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence SGSM3 Antibody - BSA Free
Western Blot SGSM3 Antibody - BSA Free
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
JunD Antibody
(2D5A1)
NBP3-16602
Immunoprecipitation JunD Antibody (2D5A1)
Western Blot JunD Antibody (2D5A1)
Immunohistochemistry JunD Antibody (2D5A1)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB

Publications for JNK1/JNK2 Antibody (MAB2076)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB2076 Applications Species
YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA Chin. Med. J., 2020-03-20;0(6):679-686. 2020-03-20 [PMID: 32068606] (Western Blot, Human) Western Blot Human
M Fang, WH Zhong, WL Song, YY Deng, DM Yang, B Xiong, HK Zeng, HD Wang Ulinastatin Ameliorates Pulmonary Capillary Endothelial Permeability Induced by Sepsis Through Protection of Tight Junctions via Inhibition of TNF-? and Related Pathways Front Pharmacol, 2018-08-13;9(0):823. 2018-08-13 [PMID: 30150933] (Western Blot, Rat) Western Blot Rat
S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response Cell Death Dis, 2017-07-27;8(7):e2955. 2017-07-27 [PMID: 28749468] (Western Blot, Human) Western Blot Human
Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress. PLoS ONE, 2014-07-28;9(7):e103148. 2014-07-28 [PMID: 25068294] (Western Blot, Mouse) Western Blot Mouse
Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis. PLoS ONE, 2013-11-22;8(11):e80971. 2013-11-22 [PMID: 24278357] (Western Blot, Human) Western Blot Human
Rajasekaran K, Kumar P, Schuldt KM et al. Signaling by Fyn-ADAP via the Carma1-Bcl-10-MAP3K7 signalosome exclusively regulates inflammatory cytokine production in NK cells. Nat Immunol. 11/1/2013 [PMID: 24036998]

Reviews for JNK1/JNK2 Antibody (MAB2076) (0)

There are no reviews for JNK1/JNK2 Antibody (MAB2076). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for JNK1/JNK2 Antibody (MAB2076) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional JNK1/JNK2 Products

Array MAB2076

Blogs on JNK1/JNK2

There are no specific blogs for JNK1/JNK2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our JNK1/JNK2 Antibody (252323) [Unconjugated] and receive a gift card or discount.