![Immunocytochemistry/Immunofluorescence: ITCH/AIP4 Antibody (01) [NBP3-06616] - Staining of Human ITCH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ITCH monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-01-Immunocytochemistry-Immunofluorescence-NBP3-06616-img0001.jpg "Immunocytochemistry/Immunofluorescence: ITCH/AIP4 Antibody (01) [NBP3-06616] - Staining of Human ITCH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ITCH monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF |
| Clone | 01 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITCH/AIP4 (Accession#: NP_113671.3; Arg526-Glu903). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ITCH |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ITCH/AIP4 Antibody (NBP3-06616)Find related products by research area.
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| Gene Symbol | ITCH |
![Flow Cytometry: ITCH/AIP4 Antibody (01) [NBP3-06616] - Analysis of Human ITCH expression on HeLa cells. The cells were treated, stained with purified anti-Human ITCH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-01-Flow-Cytometry-NBP3-06616-img0002.jpg "Flow Cytometry: ITCH/AIP4 Antibody (01) [NBP3-06616] - Analysis of Human ITCH expression on HeLa cells. The cells were treated, stained with purified anti-Human ITCH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")