![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Wild-type (WT) and IDH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-Western-Blot-NBP1-31599-img0005.jpg "Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Wild-type (WT) and IDH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, Rt, RMSpecies Glossary |
| Applications | WB, IHC, IP, KD, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Isocitrate Dehydrogenase 1/IDH1. The exact sequence is proprietary. |
| Localization | Cytoplasm, Peroxisome |
| Predicted Species | Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | IDH1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 12/11/2023 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-31599)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IDH1 |
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-Western-Blot-NBP1-31599-img0006.jpg "Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-Western-Blot-NBP1-31599-img0010.jpg "Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - A. 30 ug Neuro2A whole cell extract B. 30 ug C8D30 whole cell extractC. 30 ug NIH-3T3 whole cell extract D. 30 ug Raw 264.7 whole cell extract E. 30 ug C2Cl2 whole cell extract 10 % SDS-PAGE IDH1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-Western-Blot-NBP1-31599-img0011.jpg "Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - A. 30 ug Neuro2A whole cell extract B. 30 ug C8D30 whole cell extractC. 30 ug NIH-3T3 whole cell extract D. 30 ug Raw 264.7 whole cell extract E. 30 ug C2Cl2 whole cell extract 10 % SDS-PAGE IDH1 antibody dilution: 1:1000")
![Immunohistochemistry-Paraffin: Isocitrate dehydrogenase Antibody [NBP1-31599] - Paraffin-embedded colon ca, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Isocitrate-dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-31599-img0002.jpg "Immunohistochemistry-Paraffin: Isocitrate dehydrogenase Antibody [NBP1-31599] - Paraffin-embedded colon ca, using antibody at 1:100 dilution.")
![Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 ug of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-Immunoprecipitation-NBP1-31599-img0014.jpg "Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 ug of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31599_rabbit-polyclonal-isocitrate-dehydrogenase-1-idh1-antibody-91020242011461.jpg "Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31599_rabbit-polyclonal-isocitrate-dehydrogenase-1-idh1-antibody-910202420134086.jpg "Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31599_rabbit-polyclonal-isocitrate-dehydrogenase-1-idh1-antibody-910202420104419.jpg "Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31599_rabbit-polyclonal-isocitrate-dehydrogenase-1-idh1-antibody-9102024205624.jpg "Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31599_rabbit-polyclonal-isocitrate-dehydrogenase-1-idh1-antibody-910202420134028.jpg "Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31599_rabbit-polyclonal-isocitrate-dehydrogenase-1-idh1-antibody-910202419593536.jpg "Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Wild-type (WT) andIDH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31599_rabbit-polyclonal-isocitrate-dehydrogenase-1-idh1-antibody-1610202419405773.jpg "Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Wild-type (WT) andIDH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Flow Cytometry Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]](https://images.novusbio.com/images/antibody/mab812_human-flt-3-flk-2-mab-clone-66903-flow-cytometry-3112023131157..jpg)
![Flow Cytometry Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]](https://images.novusbio.com/images/antibody/mab812_human-flt-3-flk-2-mab-clone-66903-flow-cytometry-3112023131255..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
