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IRF4 Antibody (MUM1/8560R)

Images

 
F IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using IRF4 antibody (MUM1/8560R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, ...read more
Formalin-fixed, paraffin-embedded human tonsil stained with IRF4 antibody (MUM1/8560R). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human tonsil stained with IRF4 antibody (MUM1/8560R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
MUM1/8560R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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IRF4 Antibody (MUM1/8560R) Summary

Description
Positive Controls: Human tonsil or diffuse large B-cell lymphoma (DLBCL).

Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (corresponding to C-terminal) of IRF4 protein (exact sequence is proprietary)
Localization
Nucleus.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
IRF4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for IRF4 Antibody (MUM1/8560R)

  • interferon regulatory factor 4
  • IRF4
  • IRF-4
  • LSIRF
  • LSIRFMUM1
  • Lymphocyte-specific interferon regulatory factor
  • Multiple myeloma oncogene 1
  • MUM1
  • NF-EM5

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IRF4