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Integrin beta 2/CD18 Antibody (MEM-148) [Biotin]

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Product Details

Summary
Reactivity Hu, Po(-)Species Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
MEM-148
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-480AF350
Alexa Fluor 405
NB500-480AF405
Alexa Fluor 488
NB500-480AF488
Alexa Fluor 532
NB500-480AF532
Alexa Fluor 594
NB500-480AF594
Alexa Fluor 647
NB500-480AF647
Alexa Fluor 700
NB500-480AF700
Alexa Fluor 750
NB500-480AF750
Allophycocyanin
NB500-480APC
Allophycocyanin/Cy7
NB500-480APCCY7
CoraFluor 1
NB500-480CL1
DyLight 350
NB500-480UV
DyLight 405
NB500-480V
DyLight 488
NB500-480G
DyLight 550
NB500-480R
DyLight 594
NB500-480DL594
DyLight 650
NB500-480C
DyLight 680
NB500-480FR
DyLight 755
NB500-480IR
FITC
NB500-480F
HRP
NB500-480H
Janelia Fluor 525
NB500-480JF525
Janelia Fluor 549
NB500-480JF549
Janelia Fluor 585
NB500-480JF585
Janelia Fluor 635
NB500-480JF635
Janelia Fluor 646
NB500-480JF646
Janelia Fluor 669
NB500-480JF669
PE
NB500-480PE
PE/Atto594
NB500-480PEATT594
PE/Cy5.5
NB500-480PECY55
PE/Cy7
NB500-480PECY7
PerCP
NB500-480PCP
Unconjugated
NB500-480
mFluor Violet 450 SE
NB500-480MFV450
mFluor Violet 500 SE
NB500-480MFV500
mFluor Violet 610 SE
NB500-480MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB500-480
BSA Free
NB500-480
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Integrin beta 2/CD18 Antibody (MEM-148) [Biotin] Summary

Additional Information
Clone MEM-148 was used by HLDA to establish CD designation.
Immunogen
Human tissue / cell preparation, Peripheral blood mononuclear cells (NM_000211.3).
Specificity
Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Integrin beta 2/CD18 Antibody (MEM-148) [Biotin]

  • 959 beta subunit precursor)10
  • CD18 antigen
  • CD18
  • CD18beta 2
  • cell surface adhesion glycoprotein (LFA-1
  • CR3
  • Integrin beta 2
  • integrin beta-2
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • ITGB2
  • LAD
  • LCAMB
  • leukocyte cell adhesion molecule CD18
  • leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • LFA-1 beta
  • LFA-1
  • MAC-1 beta
  • MF17
  • MFI7
  • P150
  • p150,95 beta

Background

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.
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Bioinformatics

Gene Symbol ITGB2