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Integrin beta 1/CD29 Antibody

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Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody diluted at 1:4000. The ...read more
Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody [NBP2-16974] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human Integrin beta 1 / CD29-transfected 293T cells 7.5 % SDS-PAGEIntegrin beta 1 ...read more
Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-16974] - Human colon carcinoma, using Integrin beta 1 / CD29 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, ...read more
Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] -Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody diluted at 1:5000. The ...read more
Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1/CD29 antibody (NBP2-16974) diluted at 1:5000. The ...read more
Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-16974] - Integrin beta 1/CD29 antibody detects Integrin beta 1/CD29 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse ...read more
Genetic Strategies: Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was ...read more

Product Details

Summary
Reactivity Hu, Mu, Ca, Rt, Po, Bv, Ch, Fe, Sh, XpSpecies Glossary
Applications WB, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Integrin beta 1/CD29 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Integrin beta 1/CD29. The exact sequence is proprietary.
Localization
Cell membrane; Single-pass type I membrane protein; Melanosome
Marker
Stem Cell Marker
Predicted Species
Rat (97%), Porcine (98%), Bovine (97%), Feline (98%), Chicken (94%), Sheep (98%), Xenopus (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITGB1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) Reported in scientific literature (PMID:33867854)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-16974 in the following applications:

Reactivity Notes

Xenopus laevis (91%), Cat (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Integrin beta 1/CD29 Antibody

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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202-IL
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Publications for Integrin beta 1/CD29 Antibody (NBP2-16974)(7)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 4 applications: Chemotaxis, ICC/IF, IF/IHC, Simple Western.


Filter By Application
Chemotaxis
(1)
ICC/IF
(1)
IF/IHC
(1)
Simple Western
(1)
All Applications
Filter By Species
Rat
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-16974 Applications Species
Żaneta Dzięgelewska-Sokołowska, Alicja Majewska, Iwona Szopa, Małgorzata Gajewska, Andrea Frontini Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells International Journal of Molecular Sciences 2024-04-09 [PMID: 38673732]
Krajnak S, Jakel J, Anic K Et al. Role of integrins in the metastatic spread of high-grade serous ovarian cancer Archives of gynecology and obstetrics 2021-10-24 [PMID: 34689222]
Hlavac N, Seroski DT, Agrawal NK et al. Chondroitinase ABC/galectin-3 fusion proteins with hyaluronan-based hydrogels stabilize enzyme and provide targeted enzyme activity for neural applications Journal of neural engineering 2021-06-22 [PMID: 34082409]
Zhang W, Wang H, Yuan Z et al. Moderate mechanical stimulation rescues degenerative annulus fibrosus by suppressing caveolin-1 mediated pro-inflammatory signaling pathway International journal of biological sciences 2021-04-03 [PMID: 33867854] (IF/IHC, Chemotaxis, Rat) IF/IHC, Chemotaxis Rat
Ngo TB, Spearman BS, Hlavac N, Schmidt CE Three-Dimensional Bioprinted Hyaluronic Acid Hydrogel Test Beds for Assessing Neural Cell Responses to Competitive Growth Stimuli ACS biomaterials science & engineering 2020-12-14 [PMID: 33320621]
Bousalis D, Lacko CS, Hlavac N et al. Extracellular Matrix Disparities in an Nkx2-5 Mutant Mouse Model of Congenital Heart Disease Frontiers in Cardiovascular Medicine 2020-05-29 [PMID: 32548129] (Simple Western) Simple Western
Wu L, Xu Y, Xi K et al. Regulation of macrophage subtype via injectable micro/nano-structured porous microsphere for reprogramming osteoimmune microenvironment Chemical Engineering Journal 2022-07-01 (ICC/IF, Rat) ICC/IF Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (NBP2-16974). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Integrin beta 1/CD29 Antibody (NBP2-16974)

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits. ...  Read full blog post.

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Bioinformatics

Gene Symbol ITGB1
Uniprot