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Integrin alpha 4/CD49d Antibody (7.2R) [Unconjugated]

 (7 Publications)
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Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more
Integrin a4/ CD49d was detected in immersion fixed U937 human histiocytic lymphoma cell line using 10 µg/mL Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354) for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
7.2R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1354U-100UG
Alexa Fluor 405
FAB1354V-100UG
Alexa Fluor 488
FAB1354G
Alexa Fluor 532
FAB1354X
Alexa Fluor 594
FAB1354T-100UG
Alexa Fluor 647
FAB1354R-100UG
Alexa Fluor 700
FAB1354N-100
Alexa Fluor 750
FAB1354S-100UG
Allophycocyanin/Cy7
FAB1354APCCY7
Biotin
BAM1354
CoraFluor 1
FAB1354CL1
DyLight 350
FAB1354D
DyLight 405
FAB1354E
DyLight 488
FAB1354K
DyLight 550
FAB1354L
DyLight 594
FAB1354M
DyLight 650
FAB1354W
DyLight 680
FAB1354Y
DyLight 755
FAB1354Z
FITC
FAB1354F
HRP
FAB1354H
Janelia Fluor 525
FAB1354JF525
Janelia Fluor 549
FAB1354I
Janelia Fluor 646
FAB1354J
Janelia Fluor 669
FAB1354JF669
PE/Atto594
FAB1354PEATT594
PE/Cy5.5
FAB1354PECY55
PE/Cy7
FAB1354PECY7
Phycoerythrin
FAB1354P
mFluor Violet 450 SE
FAB1354MFV450
mFluor Violet 500 SE
FAB1354MFV500
mFluor Violet 610 SE
FAB1354MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB1354P
Carrier Free
FAB1354G
Carrier Free
MAB1354-SP
Carrier Free
MAB1354-500
Carrier Free
FAB1354N-100
Carrier Free
FAB1354V-100UG
Carrier Free
FAB1354U-100UG
Carrier Free
FAB1354T-100UG
Carrier Free
FAB1354S-100UG
Carrier Free
FAB1354R-100UG
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Integrin alpha 4/CD49d Antibody (7.2R) [Unconjugated] Summary

Immunogen
DX-3 human melanoma cell line
Specificity
Detects human Integrin alpha 4/CD49d. Recognizes the human Integrin alpha 4/CD49d subunit.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGA4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB1354 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 4/CD49d Antibody (7.2R) [Unconjugated]

  • 269C wild type
  • CD49 antigen-like family member D
  • CD49d antigen
  • CD49d
  • CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
  • IA4
  • Integrin alpha 4
  • integrin alpha-4 subunit
  • integrin alpha-4
  • Integrin alpha-IV
  • integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
  • ITGA4
  • MGC90518
  • very late activation protein 4 receptor, alpha 4 subunit
  • VLA-4 subunit alpha
  • VLA-4

Background

The alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 and alpha 4 beta 7 are receptors for fibronectin and VCAM. Integrin alpha 4 beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Integrin alpha
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Integrin alpha 4/CD49d Antibody (7.2R) [Unconjugated]
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Publications for Integrin alpha 4/CD49d Antibody (MAB1354)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Bioassay, Flow Cytometry, ICC, Neutralization.


Filter By Application
Bioassay
(1)
Flow Cytometry
(2)
ICC
(2)
Neutralization
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB1354 Applications Species
Su, Y;Luo, Z;Sun, D;Yang, B;Li, Q; The Force-Dependent Mechanism of an Integrin ?4?7-MAdCAM-1 Interaction International journal of molecular sciences 2023-11-07 [PMID: 38003252] (Flow Cytometry, Human) Flow Cytometry Human
JD Howden, M Michael, W Hight-Warb, M Parsons &alpha2&beta1 integrins spatially restrict Cdc42 activity to stabilise adherens junctions Bmc Biology, 2021-06-23;19(1):130. 6/23/2021 [PMID: 34158053]
D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells J. Clin. Invest., 2019-05-14;129(7):2745-2759. 2019-05-14 [PMID: 31085833] (Bioassay, Human) Bioassay Human
Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation. Sci Rep, 2015-03-20;5(0):9275. 2015-03-20 [PMID: 25792190] (Flow Cytometry, Human) Flow Cytometry Human
Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium. Arthritis Rheum., 2007-06-01;56(6):1817-26. 2007-06-01 [PMID: 17530710] (Neutralization, Human) Neutralization Human
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007-03-30;52(10):2775-83. 2007-03-30 [PMID: 17394066] (ICC, Human) ICC Human
Carman CV, Springer TA A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them. J. Cell Biol., 2004-10-25;167(2):377-88. 2004-10-25 [PMID: 15504916] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol ITGA4