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Integrin alpha 4/CD49d Antibody (7.2R) [Biotin]

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Integrin  alpha 4/CD49d was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Integrin  alpha 4/CD49d Biotinylated Monoclonal Antibody (Catalog # BAM1354) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Integrin  alpha 4/CD49d was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Integrin  alpha 4/CD49d Biotinylated Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
7.2R
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1354U-100UG
Alexa Fluor 405
FAB1354V-100UG
Alexa Fluor 488
FAB1354G
Alexa Fluor 532
FAB1354X
Alexa Fluor 594
FAB1354T-100UG
Alexa Fluor 647
FAB1354R-100UG
Alexa Fluor 700
FAB1354N-100
Alexa Fluor 750
FAB1354S-100UG
Allophycocyanin/Cy7
FAB1354APCCY7
CoraFluor 1
FAB1354CL1
DyLight 350
FAB1354D
DyLight 405
FAB1354E
DyLight 488
FAB1354K
DyLight 550
FAB1354L
DyLight 594
FAB1354M
DyLight 650
FAB1354W
DyLight 680
FAB1354Y
DyLight 755
FAB1354Z
FITC
FAB1354F
HRP
FAB1354H
Janelia Fluor 525
FAB1354JF525
Janelia Fluor 549
FAB1354I
Janelia Fluor 646
FAB1354J
Janelia Fluor 669
FAB1354JF669
PE/Atto594
FAB1354PEATT594
PE/Cy5.5
FAB1354PECY55
PE/Cy7
FAB1354PECY7
Phycoerythrin
FAB1354P
Unconjugated
MAB1354-100
mFluor Violet 450 SE
FAB1354MFV450
mFluor Violet 500 SE
FAB1354MFV500
mFluor Violet 610 SE
FAB1354MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1354-100
Carrier Free
FAB1354P
Carrier Free
FAB1354G
Carrier Free
MAB1354-SP
Carrier Free
MAB1354-100
Carrier Free
MAB1354-500
Carrier Free
FAB1354N-100
Carrier Free
FAB1354V-100UG
Carrier Free
FAB1354U-100UG
Carrier Free
FAB1354T-100UG
Carrier Free
FAB1354S-100UG
Carrier Free
FAB1354R-100UG
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Integrin alpha 4/CD49d Antibody (7.2R) [Biotin] Summary

Immunogen
DX-3 human melanoma cell line
Specificity
Detects human Integrin alpha 4/CD49d.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGA4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Readout System
Publications
Read Publication using
BAM1354 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 4/CD49d Antibody (7.2R) [Biotin]

  • 269C wild type
  • CD49 antigen-like family member D
  • CD49d antigen
  • CD49d
  • CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
  • IA4
  • Integrin alpha 4
  • integrin alpha-4 subunit
  • integrin alpha-4
  • Integrin alpha-IV
  • integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
  • ITGA4
  • MGC90518
  • very late activation protein 4 receptor, alpha 4 subunit
  • VLA-4 subunit alpha
  • VLA-4

Background

The alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 and alpha 4 beta 7 are receptors for fibronectin and VCAM. Integrin alpha 4 beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Integrin alpha
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Integrin alpha 4/CD49d Antibody (7.2R) [Biotin]
Species: Hu
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Publications for Integrin alpha 4/CD49d Antibody (BAM1354)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol ITGA4