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Integrin alpha 2/CD49b Antibody - Azide and BSA Free

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Genetic Strategies: Western Blot: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Western blot analysis of extracts from normal (control) and Integrin alpha 2/CD49b knockout (KO) 293T cells, using Integrin alpha ...read more
Immunocytochemistry/ Immunofluorescence: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Analysis of U2OS cells using Integrin alpha 2/CD49b antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] Integrin alpha 2/CD49b Rabbit pAb (NBP3-03851) at dilution of ...read more
Western Blot: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Western blot analysis of extracts of various cell lines, using Integrin alpha 2/CD49b antibody (NBP3-03851) at 1:1000 dilution. Secondary antibody: HRP Goat ...read more
Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Immunohistochemistry of paraffin-embedded human lung cancer using [KO Validated] Integrin alpha 2/CD49b Rabbit pAb (NBP3-03851) at dilution ...read more
Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] Integrin alpha 2/CD49b Rabbit pAb (NBP3-03851) at dilution of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Validated by:
     

Genetic Strategies

   

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Integrin alpha 2/CD49b Antibody - Azide and BSA Free Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 30-162 of human Integrin alpha 2/CD49b (NP_002194.2). YNVGLPEAKIFSGPSSEQFGYAVQQFINPKGNWLLVGSPWSGFPENRMGDVYKCPVDLSTATCEKLNLQTSTSIPNVTEMKTNMSLGLILTRNMGTGGFLTCGPLWAQQCGNQYYTTGVCSDISPDFQLSASF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITGA2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:500 - 1:1000
Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Integrin alpha 2/CD49b Antibody - Azide and BSA Free

  • alpha-2 subunit
  • CD49b antigen
  • CD49b
  • Integrin alpha 2
  • integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
  • ITGA2
  • VLA 2
  • VLA-2 alpha

Background

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin alpha 2/CD49b Antibody (NBP3-03851) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ITGA2