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Mouse Insulin ELISA Kit (Colorimetric)

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Mouse Insulin ELISA Kit (Colorimetric) [NBP3-18131] - Samples were spiked with high concentrations of Mouse Insulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Mouse Insulin ELISA Kit (Colorimetric) [NBP3-18131] - Samples were spiked with high concentrations of Mouse Insulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Mouse Insulin ELISA Kit (Colorimetric) [NBP3-18131] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma
Standard Curve Range
3.13-200 pg/ml
Sensitivity
1.88 pg/ml

Order Details

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Mouse Insulin ELISA Kit (Colorimetric) Summary

Description
Higher sensitivity and precision than competitors.
Specificity
No significant crossreactivity or interference between Mouse Insulin and analogues was observed
Standard Curve Range
3.13-200 pg/ml
Sensitivity
1.88 pg/ml
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.46
Intra-Assay
CV% < 5.56
Spike Recovery
85-98%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
INS

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate(Dismountable)
  7. Plate Sealer
  8. Product Description
  9. Reference Standard & Sample Diluent
  10. Reference Standard
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Insulin ELISA Kit (Colorimetric)

  • IDDM2
  • ILPR
  • INS
  • Insulin
  • IRDN
  • MODY10
  • proinsulin

Background

Insulin is a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as a-cell of islets of Langerhans and tumor (insulinoma) marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Insulin ELISA Kit (NBP3-18131). (Showing 1 - 1 of 1 FAQ).

  1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
    • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

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Bioinformatics

Gene Symbol INS