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Recombinant Mouse IL-9 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Mouse IL-9 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 126 amino acids corresponding to IL-9 Source: E. coli

Uniprot ID: P15247

Amino Acid Sequence: QRCSTTWGIR DTNYLIENLK DDPPSKCSCS GNVTSCLCLS VPTDDCTTPC YREGLLQLTN ATQKSRLLPV FHRVKRIVEV LKNITCPSFS CEKPCNQTMA GNTLSFLKSL LGTFQKTEMQ RQKSRP
Details of Functionality
IL-9 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine TS1 cells is less than 0.02 ng/ml, corresponding to a specific activity of > 5.0 x 10^7 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL9
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of IL-9 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
14.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Mouse IL-9 Protein

  • Cytokine P40
  • HP40
  • IL9
  • IL-9
  • IL-9homolog of mouse T cell and mast cell growth factor 40
  • interleukin 9
  • interleukin-9
  • p40 cytokine
  • p40 T-cell and mast cell growth factor
  • P40
  • T-cell growth factor P40

Background

IL-9 is a cytokine with immunoregulatory function, produced by activated T-helper type 2 lymphocytes. It acts on various cell types such as T- and B-lymphocytes, mast cells, haemopoietic progenitors and megakaryoblastic leukemia cell lines. IL-9 stimulates cell proliferation and prevents apoptosis. IL-9 may play a role in the pathogenesis of diseases such as certain lymphomas (Hodgin's disease, anaplastic lymphoma) and asthma. Although murine IL-9 can act on human cells, human IL-9 is inactive on mouse cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL9