IL-9 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

IL-9 is a cytokine with immunoregulatory function, produced by activated T-helper type 2 lymphocytes. It acts on various cell types such as T- and B-lymphocytes, mast cells, haemopoietic progenitors and megakaryoblastic leukemia cell lines. IL-9 stimulates cell proliferation and prevents apoptosis. IL-9 may play a role in the pathogenesis of diseases such as certain lymphomas (Hodgin's disease, anaplastic lymphoma) and asthma. Although murine IL-9 can act on human cells, human IL-9 is inactive on mouse cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-11970

• IL-9 Antibodies
• IL-9 ELISA Kit
• IL-9 Lysate
• IL-9 Proteins
• IL-9 Proteins and Enzymes
• IL-9 ELISAs