Reactivity | MuSpecies Glossary |
Applications | Flow |
Clone | 222604 |
Clonality | Monoclonal |
Host | Rat |
Conjugate | Alexa Fluor 405 |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑9 Gln19-Pro144 Accession # P15247.1 |
Specificity | Detects mouse IL-9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human IL-9 is observed. |
Isotype | IgG2a |
Clonality | Monoclonal |
Host | Rat |
Gene | IL9 |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative | 0.09% Sodium Azide |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Mouse IL-9 was originally identified as a T cell-derived T cell growth factor III/P40 which could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. IL-9 can also prolong the in vitro survival of other T cell clones as well as potentiate the IL-2 dependent proliferation of mouse fetal thymocytes. However, this cytokine has no growth-stimulating activity on mouse cytolytic T cell clones or fresh T cells. Mouse IL-9 also has mast cell enhancing activity (MEA) and can enhance the mIL-3- or mIL-4-dependent proliferation of mouse bone marrow-derived mast cells. Furthermore, IL-9 will synergize with erythropoietin to support erythroid colony formation in vitro. The mouse IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four potential N-linked glycosylation sites and the native mIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% amino acid sequence homology. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells.
Secondary Antibodies |
Isotype Controls |
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