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Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

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ELISA: Human IL-7R alpha/CD127 ELISA Kit (Colorimetric) [NBP3-06768] - Samples were spiked with high concentrations of Human IL-7R alpha/CD127 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human IL-7R alpha/CD127 ELISA Kit (Colorimetric) [NBP3-06768] - Samples were spiked with high concentrations of Human IL-7R alpha/CD127 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human IL-7R alpha/CD127 ELISA Kit (Colorimetric) [NBP3-06768] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

Order Details

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Human IL-7R alpha/CD127 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human IL-7R alpha/CD127 in samples. No significant cross-reactivity or interference between Human IL-7R alpha/CD127 and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.36
Intra-Assay
%CV < 5.39
Spike Recovery
87 - 111%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL7R

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Background

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human IL-7R
alpha/CD127 ELISA Kit ...
NBP3-06768
Human IL-7R alpha/CD127 ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

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Product PDFs

  SDS
  Human IL-7R alpha/CD127 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol IL7R