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IL-7R alpha/CD127 Antibody (717519) [Unconjugated]

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CD4+rat splenocytes were stained with Mouse Anti-Rat IL-7 R alpha /CD127 Monoclonal Antibody (Catalog # MAB8484, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
CD4+rat splenocytes were stained with Mouse Anti-Rat IL-7 R alpha /CD127 Monoclonal Antibody (Catalog # MAB8484, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by anti-Mouse ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, CyTOF-ready
Clone
717519
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8484U-100UG
Alexa Fluor 405
FAB8484V-100UG
Alexa Fluor 488
FAB8484G-100UG
Alexa Fluor 532
FAB8484X
Alexa Fluor 594
FAB8484T-100UG
Alexa Fluor 647
FAB8484R-100UG
Alexa Fluor 700
FAB8484N-100UG
Alexa Fluor 750
FAB8484S-100UG
Allophycocyanin
FAB8484A
Allophycocyanin/Cy7
FAB8484APCCY7
Biotin
FAB8484B
CoraFluor 1
FAB8484CL1
DyLight 350
FAB8484D
DyLight 405
FAB8484E
DyLight 488
FAB8484K
DyLight 550
FAB8484L
DyLight 594
FAB8484M
DyLight 650
FAB8484W
DyLight 680
FAB8484Y
DyLight 755
FAB8484Z
FITC
FAB8484F
HRP
FAB8484H
Janelia Fluor 525
FAB8484JF525
Janelia Fluor 549
FAB8484I
Janelia Fluor 585
FAB8484JF585
Janelia Fluor 635
FAB8484JF635
Janelia Fluor 646
FAB8484J
Janelia Fluor 669
FAB8484JF669
PE/Atto594
FAB8484PEATT594
PE/Cy5.5
FAB8484PECY55
PE/Cy7
FAB8484PECY7
PerCP
FAB8484C
Phycoerythrin
FAB8484P-100
Phycoerythrin
FAB8484P-025
mFluor Violet 450 SE
FAB8484MFV450
mFluor Violet 500 SE
FAB8484MFV500
mFluor Violet 610 SE
FAB8484MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB8484P-025
Carrier Free
MAB8484-SP
Carrier Free
FAB8484S-100UG
Carrier Free
FAB8484U-100UG
Carrier Free
FAB8484V-100UG
Carrier Free
FAB8484R-100UG
Carrier Free
FAB8484N-100UG
Carrier Free
FAB8484T-100UG
Carrier Free
FAB8484G-100UG
Carrier Free
FAB8484P-100
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IL-7R alpha/CD127 Antibody (717519) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127
Glu21-Asp239
Accession # NP_001099888
Specificity
Detects rat IL‑7 R alpha /CD127in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-7R alpha/CD127 Antibody (717519) [Unconjugated]

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Background

IL-7 R alpha (IL-7 receptor alpha; also CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 aa type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129‑222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21‑239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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