Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL‑7 R alpha /CD127 Monoclonal Antibody ...read more
Steroid resistance induced by wild-type or mutant IL7R signaling molecules is associated with activation of MEK-ERK and/or AKT.(A) Western blot results for total and/or phosphorylated levels of IL7R signaling molecules ...read more
Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha Extracellular domain
Specificity
Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human (rh) IL‑10 R, rhIL‑2 R beta , rhIL‑5 R alpha , and rhIL‑6 R.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL7R
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IL-7R alpha/CD127 Antibody (40131) [Unconjugated]
CD127 antigen
CD127
CD127ILRA
CDW127
IL-7 R alpha
IL-7 receptor subunit alpha
IL7R alpha
IL-7R alpha
IL-7R subunit alpha
IL7R
IL7RA
IL-7Ra
IL-7R-alpha
interleukin 7 receptor alpha chain
interleukin 7 receptor isoform H5-6
interleukin 7 receptor
interleukin-7 receptor subunit alpha
Background
Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha . IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).
Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.
Goodwin, R.G. et al. (1990) Cell 60:941.
Noguchi, M. et al. (1993) Science 262:1877.
Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90:9125.
Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348.
Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Reche, P.A. et al. (2001) J. Immunol. 167:336.
Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
Jahn, T. et al. (2007) Blood 110:1840.
Lai, L. et al. (2006) Blood 107:1776.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for IL-7R alpha/CD127 Antibody (MAB306)(18)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 7 applications: ELISA Capture, ELISA Development, Flow Cytometry, ICC, Immunoprecipitation, Neutralization, Western Blot.
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