Home » IL-7R alpha/CD127 » IL-7R alpha/CD127 Antibodies » IL-7R alpha/CD127 Antibody (22F3D9) [PE]
Novus Biologicals products are now on bio-techne.com

IL-7R alpha/CD127 Antibody (22F3D9) [PE]

 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Flow Cytometry: IL-7 R alpha/CD127 Antibody (22F3D9) [PE] [NBP2-22376PE] - analysis of IL7 Receptor alpha in human PBMC using anti-IL7 Receptor alpha antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 minutes at 4C. Image from verified customer review.
Flow Cytometry: IL-7 R alpha/CD127 Antibody (22F3D9) [PE] [NBP2-22376PE] - analysis of IL7 Receptor alpha in human PBMC using anti-IL7 Receptor alpha antibody. The primary antibody was used at a dilution of 1:100, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
22F3D9
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22376AF350
Alexa Fluor 405
NBP2-22376AF405
Alexa Fluor 488
NBP2-22376AF488
Alexa Fluor 532
NBP2-22376AF532
Alexa Fluor 594
NBP2-22376AF594
Alexa Fluor 647
NBP2-22376AF647
Alexa Fluor 700
NBP2-22376AF700
Alexa Fluor 750
NBP2-22376AF750
Allophycocyanin
NBP2-22376APC
Allophycocyanin/Cy7
NBP2-22376APCCY7
Biotin
NBP2-22376B
CoraFluor 1
NBP2-22376CL1
DyLight 350
NBP2-22376UV
DyLight 405
NBP2-22376V
DyLight 488
NBP2-22376G
DyLight 550
NBP2-22376R
DyLight 594
NBP2-22376DL594
DyLight 650
NBP2-22376C
DyLight 680
NBP2-22376FR
DyLight 755
NBP2-22376IR
FITC
NBP2-22376F
HRP
NBP2-22376H
Janelia Fluor 525
NBP2-22376JF525
Janelia Fluor 549
NBP2-22376JF549
Janelia Fluor 646
NBP2-22376JF646
Janelia Fluor 669
NBP2-22376JF669
PE/Atto594
NBP2-22376PEATT594
PE/Cy5.5
NBP2-22376PECY55
PE/Cy7
NBP2-22376PECY7
PerCP
NBP2-22376PCP
Unconjugated
NBP2-22376
mFluor Violet 450 SE
NBP2-22376MFV450
mFluor Violet 500 SE
NBP2-22376MFV500
mFluor Violet 610 SE
NBP2-22376MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-22376
BSA Free
NBP2-22376
BSA Free
NBP2-22376SS
Azide and BSA Free
NBP2-80812
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

IL-7R alpha/CD127 Antibody (22F3D9) [PE] Summary

Immunogen
Amino acids 141-237 of human CD127 were used as the immunogen for the antibody.
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
IL7R
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22376PE in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for IL-7R alpha/CD127 Antibody (22F3D9) [PE]

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Background

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CD25/IL-2R alpha
[HRP]
DR2A00
N/A CD25/IL-2R alpha [HRP]
N/A CD25/IL-2R alpha [HRP]
(36 Publications)
Species: Hu
Applications: ELISA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
IL-7 [Unconjugated]
207-IL
SEC-MALS IL-7 [Unconjugated]
(157 Publications)
Species: Hu
Applications: BA
ISG20 Antibody
(1B2-3C9)
H00003669-M01
Western Blot ISG20 Antibody (1B2-3C9)
Western Blot ISG20 Antibody (1B2-3C9)
Immunocytochemistry/ Immunofluorescence ISG20 Antibody (1B2-3C9)
(1 Review)
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
FoxP3 Antibody -
BSA Free
NB100-39002
Flow Cytometry FoxP3 Antibody - BSA Free
Flow Cytometry FoxP3 Antibody - BSA Free
Immunohistochemistry-Paraffin FoxP3 Antibody - BSA Free
(2 Reviews)
(62 Publications)
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(302 Publications)
Species: Mu
Applications: ELISA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
IL-15
[Unconjugated]
247-ILB
SEC-MALS IL-15 [Unconjugated]
(165 Publications)
Species: Hu
Applications: BA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(455 Publications)
Species: Hu
Applications: BA
PD-1 Antibody
[Unconjugated]
AF1086
Western Blot PD-1 Antibody [Unconjugated]
Immunohistochemistry PD-1 Antibody [Unconjugated]
Western Blot PD-1 Antibody [Unconjugated]
(1 Review)
(65 Publications)
Species: Hu
Applications: Block, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, WB
L-Selectin/CD62L
Antibody (4G8) [Uncon ...
BBA24
Flow Cytometry L-Selectin/CD62L Antibody (4G8) [Unconjugated]
(9 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow
CD3 Antibody (SP7)
NB600-1441
Immunohistochemistry-Frozen CD3 Antibody (SP7)
Immunohistochemistry-Paraffin CD3 Antibody (SP7)
Immunohistochemistry-Paraffin CD3 Antibody (SP7)
(3 Reviews)
(45 Publications)
Species: Ca, Hu, Mu, Po
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
Common gamma
Chain/IL-2 R gamma An ...
AF284
Neutralization Common gamma Chain/IL-2 R gamma Antibody [Unconjugated]
(6 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, Neut, WB
IL-7R alpha/CD127
Antibody (22F3D9) [PE ...
NBP2-22376PE
IL-7R alpha/CD127 Antibody (22F3D9) [PE]
Species: Hu
Applications: WB, Flow, CyTOF-ready
Supplier Logo

Publications for IL-7R alpha/CD127 Antibody (NBP2-22376PE) (0)

There are no publications for IL-7R alpha/CD127 Antibody (NBP2-22376PE).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for IL-7R alpha/CD127 Antibody (NBP2-22376PE) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-22376PE:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry IL-7R alpha/CD127 NBP2-22376PE
Enlarge 		5
reviewed by:
Verified Customer
 Flow Human 11/18/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-7R alpha/CD127 Antibody (NBP2-22376PE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IL-7R alpha/CD127 Products

Research Areas for IL-7R alpha/CD127 Antibody (NBP2-22376PE)

Find related products by research area.

Blogs on IL-7R alpha/CD127

There are no specific blogs for IL-7R alpha/CD127, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0
Flow Cytometry IL-7R alpha/CD127 NBP2-22376PE
Enlarge
 Verified Customer
11/18/2014
Application: Flow
Species: Human
read this review

Bioinformatics

Gene Symbol IL7R