![Western Blot: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL3.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Western-Blot-NBP2-71023-img0001.jpg "Western Blot: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL3.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI3B11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 20-152 of human IL-3 (NP_000579) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IL3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for IL-3 Antibody (NBP2-71023)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IL3 |
![Immunocytochemistry/Immunofluorescence: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of A549 cells using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71023-img0002.jpg "Immunocytochemistry/Immunofluorescence: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of A549 cells using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0011.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0003.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Human breast tissue using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0004.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Human breast tissue using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Human colon tissue using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0005.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Human colon tissue using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Human Kidney tissue using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0006.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Human Kidney tissue using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0007.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0008.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0009.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0010.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL3 mouse monoclonal antibody.")
![Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Human breast tissue.](http://images.novusbio.com/fullsize/IL-3-Antibody-OTI3B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71023-img0012.jpg "Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Human breast tissue.")
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/DATA_Erythropoietin_MEP00_ELISA_970.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/Erythropoietin_MEP00_ELISA_421.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/IL-5_M5000_ELISA_237.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/DATA_IL5_M5000_ELISA_935.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Neutralization G-CSF Antibody (67604) [Unconjugated]](https://images.novusbio.com/images2/G-CSF_MAB414_Block_Neutralize_9188.jpg)
![Immunohistochemistry CD34 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD34_AF4117_Immunohistochemistry_6824.jpg)
![Bioactivity Thrombopoietin/THPO [Unconjugated]](https://images.novusbio.com/images/protein/288-tpncf_recombinant-human-thrombopoietin-ns0-expressed-protein-cf-bioactivity-272020135047.jpg)
![SDS-PAGE IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images2/IL-1_beta_201-LB_304.jpg)
![Bioactivity IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images2/IL-1_beta_201-LB_306.jpg)
![SEC-MALS IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images/201-lb_recombinant-human-il-1-beta-il-1f2-protein-sec-mals-123202491556.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![N/A TIMP-1 [HRP]](https://images.novusbio.com/images2/DATA_TIMP1_DTM100_ELISA_847.jpg)
![N/A TIMP-1 [HRP]](https://images.novusbio.com/images2/TIMP-1_DTM100_ELISA_194.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")