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IL-24 Antibody

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Western Blot: IL-24 Antibody [NB100-56407] - Analysis of IL-24 in the A) absence and B) presence of blocking peptide in Jurkat cell lysate using this antibody at 0.5 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: IL-24 Antibody [NB100-56407] - Analysis of IL-24 in the A) absence and B) presence of blocking peptide in Jurkat cell lysate using this antibody at 0.5 ug/ml. Goat anti-rabbit IgG HRP secondary antibody ...read more
Flow Cytometry: IL-24 Antibody [NB100-56407] - Cell surface flow cytometry analysis of IL-24 in Jurkat cells using this antibody at 5 ug/10^6 cells. The shaded histogram represents cells only, green represents isotype ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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IL-24 Antibody Summary

Immunogen
Amino acids 123-136 (KNYHNRTVEVRTLK) of human IL-24 were used as the immunogen for this antibody.
Specificity
The amino acid sequence used as immunogen is found in many of the IL-24 isoforms, including isoforms of 219, 207, 206, 154, 143 and 120 amino acids.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL24
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 2-5 ug/10^6 cells
  • Western Blot 0.5-3 ug/ml
Control
Jurkat Whole Cell Lysate
IL-24 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IL-24 Antibody

  • C49A
  • FISP
  • IL10B
  • IL24
  • IL-24
  • interleukin 24
  • MDA-7
  • MDA7MOB5
  • melanocyte-associated Mda-7
  • melanoma differentiation association protein 7
  • Melanoma differentiation-associated gene 7 protein
  • MOB-5
  • ST16
  • ST16interleukin-24
  • suppression of tumorigenicity 16 (melanoma differentiation)
  • Suppression of tumorigenicity 16 protein

Background

Interleukin 24 (IL-24)/melanoma differentiation-associated gene-7 (mda-7) encodes a secreted protein that exhibits significant homology to the interleukin 10 (IL-10) family of cytokines. The human IL-24 is secreted by activated peripheral blood mononuclear cells and binds two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2 resulting in the activation of JAK/STAT signaling pathways. Mda-7/IL-24, selectively induces growth suppression, apoptosis and radio sensitization in malignant gliomas in a p53-independent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-24 Antibody
NB100-56407
IL-24 Antibody
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Applications: WB, Flow
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Video Protocols

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Control Lysate(s)

Jurkat Whole Cell Lysate
IL-24 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL24
Entrez