Reactivity | RtSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 129 amino acids corresponding to IL-21 Source: E. coli Uniprot ID: A3QPB9 Amino Acid Sequence: HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS |
Details of Functionality | IL21 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 10^4 IU/mg. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | IL21 |
Purity | >95%, by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of IL-21 as determined by LAL method. |
Dilutions |
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Theoretical MW | 15.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | >95%, by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
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Gene Symbol | IL21 |