IL-21 Antibodies
We offer IL-21 Antibodies for use in common research applications: ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Neutralization, Proximity Ligation Assay, Western Blot. Each IL-21 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our IL-21 Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Human, Mouse, Porcine, Rat. Use the list below to choose the IL-21 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our IL-21 polyclonal antibodies and browse our IL-21 monoclonal antibody catalog.
Alternate Names for IL-21 Antibodies
anti-IL-21 antibody, anti-IL21 antibody, anti-IL-21Za11interleukin-21 antibody, anti-interleukin 21 antibody, anti-interleukin-21 isoform antibody
20 Results for "il-21" in Primary Antibodies
![Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336] - Analysis of IL-21. Full-length recombinant IL-21 protein probed with 1 ug/ml of IL-21 monoclonal antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-21-Antibody-14k5H3-Western-Blot-NBP2-27336-img0002.jpg "Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336] - Analysis of IL-21. Full-length recombinant IL-21 protein probed with 1 ug/ml of IL-21 monoclonal antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0007.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Expression of Tagged Proteins Validation. </b>Immunocytochemistry/Immunofluorescence: IL-21 Antibody [NBP1-76740] - Immunocytochemistry Validation of IL-21 in K562 Cells.Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (NBP1-76740) at 2 ug/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IL-21-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76740-img0008.jpg)
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740 (1 ug/mL), IL-21 from Company B (5 ug/mL), beta-actin (5 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0005.jpg)
![Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Figure 3 Western Blot Validation in Human HL-60 Cell LysateLoading: 15 u of lysates per lane.Antibodies: IL-21 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-76740_rabbit-polyclonal-il-21-antibody-271120241937752.jpg)
![ELISA: IL-21 Antibody [NBP3-11321] - Recombinant Rabbit IL-21 detected using Chicken anti Rabbit IL-21 (AHP2826) as the capture reagent and Chicken anti Rabbit IL-21:Biotin as the detection reagent followed by Streptavidin:HRP.](http://images.novusbio.com/fullsize/IL-21-Antibody-ELISA-NBP3-11321-img0001.jpg "ELISA: IL-21 Antibody [NBP3-11321] - Recombinant Rabbit IL-21 detected using Chicken anti Rabbit IL-21 (AHP2826) as the capture reagent and Chicken anti Rabbit IL-21:Biotin as the detection reagent followed by Streptavidin:HRP.")
![Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.](http://images.novusbio.com/fullsize/IL-21-Antibody-MT216G-21-3m-Neutralization-NBP3-18290-img0001.jpg "Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.")