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Human IL-21 ELISA Kit (Colorimetric)

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ELISA: IL-21 ELISA Kit [NBP1-84816] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate), buffered solution
Standard Curve Range
78 - 5,000 pg/mL
Sensitivity
20.0 pg/mL

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Human IL-21 ELISA Kit (Colorimetric) Summary

Description
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Specificity
The assay detects both natural and recombinant human IL-21. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.
Standard Curve Range
78 - 5,000 pg/mL
Sensitivity
20.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.7%
Intra-Assay
CV% < 5.8%
Spike Recovery
The mean recovery for sera was 71%, for plasma (EDTA) 42%, for plasma (citrate) 58% and for cell culture supernatant 95%.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL21

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-21
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human IL-21 monoclonal antibody
  5. Human IL-21 Standard lyophilized, 10 ng/mL upon reconstitution
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human IL-21 ELISA Kit (Colorimetric)

  • CVID11
  • IL21
  • IL-21
  • IL-21Za11interleukin-21
  • interleukin 21
  • interleukin-21 isoform
  • Za11

Background

The human IL-21 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-21. Interleukin-21 is a member of the type-I cytokine family. Structurally, IL-21 shows homology to IL-2, IL-4, and IL-15 proteins. IL-21 shares the common gamma-chain with these three cytokines, but, in addition, binds to a unique IL-21R alpha chain, and activates the JAK/STAT pathway. IL-21 has pleiotropic actions, from augmenting the proliferation of T cells and driving the differentiation of B cells into memory cells and terminally differentiated plasma cells to augmenting the activity of natural killer cells. IL-21 is mainly produced by activated T-cells, but targets a broad range of lymphoid and myeloid cells of the immune system and therefore is able to regulate innate and acquired immune responses. IL-21 has pathologic function in immune-inflammatory diseases. Data suggest the existence of a positive autocrine loop that could help to amplify and stabilize IL-21-driven, T cell-mediated responses. Moreover, it has antitumour activity and may have a role in the development of autoimmunity. IL-21 may have therapeutic potentials as an antitumor agent in the clinic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Bioinformatics

Gene Symbol IL21