Home » IL-12/IL-23 p40 » IL-12/IL-23 p40 Antibodies » IL-12/IL-23 p40 Antibody (C17.8) [Unconjugated]
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IL-12/IL-23 p40 Antibody (C17.8) [Unconjugated]

 (9 Publications)
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Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND50 is typically0.04-0.3 µg/mL.
Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
C17.8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4991-SP
Carrier Free
MAB4991-500
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IL-12/IL-23 p40 Antibody (C17.8) [Unconjugated] Summary

Immunogen
Recombinant mouse IL-12 p70 heterodimer
Specificity
Detects the mouse IL-12/IL-23 p40 subunit. Polymorphisms exist in the mouse p40 sequences [Ymer, S. et al. (2002) Genes and Immunity 3:151.].
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Il12b
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.04-0.3 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Mouse IL-12/IL-23 p40 Biotinylated Antibody (Catalog number BAF499)
Standard: Recombinant Mouse IL-12/IL-23 p40 Homodimer (Catalog number 499-ML)
Publications
Read Publications using
MAB4991 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-23 p40 Antibody (C17.8) [Unconjugated]

  • CLMF p40
  • CLMF
  • CLMF2
  • Cytotoxic lymphocyte maturation factor 40 kDa subunit
  • IL12 p40
  • IL-12 p40
  • IL-12 subunit p40
  • IL12B
  • IL-12B
  • IL-12BNK cell stimulatory factor chain 2
  • interleukin 12, p40
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
  • interleukin-12 beta chain
  • interleukin-12 subunit beta
  • natural killer cell stimulatory factor, 40 kD subunit
  • NKSF
  • NKSF2
  • NKSF2IL12, subunit p40

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.

IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.

The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-12/IL-23 p40
Antibody (C17.8) [Unc ...
MAB4991
IL-12/IL-23 p40 Antibody (C17.8) [Unconjugated]
Species: Mu
Applications: WB, B/N, ELISA(Cap)

Publications for IL-12/IL-23 p40 Antibody (MAB4991)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA Development, Flow Cytometry, Functional Assay, In Vivo, Neutralization.


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ELISA Development
(2)
Flow Cytometry
(1)
Functional Assay
(1)
In Vivo
(1)
Neutralization
(3)
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Human
(1)
Mouse
(7)
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Showing Publications 1 - 9 of 9.
Publications using MAB4991 Applications Species
Soon-Oh Hong, Joonsung Kim, Sungmin Lee, Jaeil Shin, Hwanjun Choi, Eunjin Lee et al. Transgenic viral expression of PH-20, IL-12, and sPD1-Fc enhances immune cell infiltration and anti-tumor efficacy of an oncolytic virus Mol Ther Oncolytics 9/21/2023 [PMID: 37727704]
Y Chen, SK Chauhan, C Shao, M Omoto, T Inomata, R Dana IFN-?-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity J. Immunol., 2017-06-21;0(0):. 2017-06-21 [PMID: 28637904] (In Vivo, Mouse) In Vivo Mouse
Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections. Am J Pathol, 2014-12-06;185(2):420-31. 2014-12-06 [PMID: 25485859] (Functional Assay, Mouse) Functional Assay Mouse
DePaolo RW, Abadie V, Tang F Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens. Nature, 2011-02-09;471(7337):220-4. 2011-02-09 [PMID: 21307853] (Neutralization, Mouse) Neutralization Mouse
Bourgeois E, Van LP, Samson M, Diem S, Barra A, Roga S, Gombert JM, Schneider E, Dy M, Gourdy P, Girard JP, Herbelin A The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production. Eur. J. Immunol., 2009-04-01;39(4):1046-55. 2009-04-01 [PMID: 19266498] (Neutralization, Mouse) Neutralization Mouse
Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche. Cancer Res., 2008-12-01;68(23):9754-62. 2008-12-01 [PMID: 19047154] (Neutralization, Mouse) Neutralization Mouse
An HJ, Jeong HJ, Um JY, Kim HM, Hong SH Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. J Ethnopharmacol, 2006-03-10;106(3):418-24. 2006-03-10 [PMID: 16530364] (ELISA Development, Mouse) ELISA Development Mouse
Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood, 2004-05-13;104(5):1404-10. 2004-05-13 [PMID: 15142882] (ELISA Development, Human) ELISA Development Human
Oppmann , B, Lesley , R, Blom , B, Timans , J C, Xu , Y, Hunte , B, Vega , F, Yu , N, Wang , J, Singh , K, Zonin , F, Vaisberg , E, Churakova , T, Liu , M, Gorman , D, Wagner , J, Zurawski , S, Liu , Y, Abrams , J S, Moore , K W, Rennick , D, de Waal-Malefyt , R, Hannum , C, Bazan , J F, Kastelein , R A Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity, 2000-11-01;13(5):715-25. 2000-11-01 [PMID: 11114383] (Flow Cytometry, Mouse) Flow Cytometry Mouse

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Bioinformatics

Gene Symbol Il12b