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IkB-alpha Antibody Pair [HRP]

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Sandwich ELISA: IkB-alpha Antibody Pair [HRP] [NBP2-79383] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Sandwich ELISA: IkB-alpha Antibody Pair [HRP] [NBP2-79383] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP
Standard Curve Range
12.5-800 pg/ml (example only; lot dependent)
Sensitivity
12.5 pg/ml (example only; lot dependent)

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
HRP
NBP2-79383-5Plates
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IkB-alpha Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human IkB-alpha.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IkB-alpha.
Standard Curve Range
12.5-800 pg/ml (example only; lot dependent)
Sensitivity
12.5 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Gene
NFKBIA

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Mouse Monoclonal Detection Antibody (HRP-conjugated)
  3. Standard

Alternate Names for IkB-alpha Antibody Pair [HRP]

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NFKBIA