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Donkey anti-Sheep IgG Secondary Antibody [HRP Polymer]

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Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) with Anti-Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). View our protocol for the VisUCyte HRP Polymer Detection Reagent.
Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity ShSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP Polymer

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
F0127
Biotin
BAF016
Fluorescein
F0125
HRP
HAF016
HRP Polymer
VC006-050
HRP Polymer
VC006-125
HRP Polymer
VC006-250
NL493
NL012
NL557
NL010
NL637
NL011
PerCP (Peridinin-chlorophyll Protein Complex)
F0128
Phycoerythrin
F0126
Unconjugated
BAF016
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
BAF016
Carrier Free
F0125
Carrier Free
F0126
Carrier Free
F0127
Carrier Free
HAF016
Carrier Free
D-501-C-ABS
Carrier Free
F0128
Carrier Free
NL011
Carrier Free
NL012
Carrier Free
NL010
Carrier Free
VC006-050
Carrier Free
VC006-125
Carrier Free
VC006-250
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Donkey anti-Sheep IgG Secondary Antibody [HRP Polymer] Summary

Immunogen
Sheep IgG
Specificity
Detects heavy ( gamma ) chains on sheep IgG and light chains on all sheep immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin sheep serum proteins or IgG from human, mouse or rabbit, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry
Publications
Read Publication using
VC006 in the following applications:

  • IF
    1 publication

Packaging, Storage & Formulations

Storage
6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a solution in PBS with preservative.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Sheep IgG Secondary Antibody [HRP Polymer]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (VC006)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF.


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