![Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2 stained by IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249355530.jpg "Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2 stained by IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:500. Blue: Fluoroshield with DAPI .")
| Reactivity | Hu, Mu, Po, CaSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | HL1982 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human IGF-II/IGF2. |
| Predicted Species | Porcine (92%), Canine (95%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | IGF2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Western Blot IGF-I R/IGF1R Antibody (33255) [Unconjugated]](https://images.novusbio.com/images2/IGF-I_R_MAB391_Western_Blot_6136.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody (33255) [Unconjugated]](https://images.novusbio.com/images2/IGFI_R_MAB391_Immunohistochemistry_21743.jpg)
![Flow Cytometry IGF-I R/IGF1R Antibody (33255) [Unconjugated]](https://images.novusbio.com/images2/IGFI_R_MAB391_Flow_Cytometry_22536.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for IGF-II/IGF2 Antibody (NBP3-25520)Find related products by research area.
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| Gene Symbol | IGF2 |
![Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249425473.jpg "Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249413315.jpg "Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Mouse plasma (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249371569.jpg "Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Mouse plasma (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL1982] (NBP3-25520) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-212024941489.jpg "ELISA: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL1982] (NBP3-25520) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25520_rabbit-igf-ii-igf2-mab-hl1982-azide-and-bsa-free-2120249355596.jpg "Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
![Mass Spectrometry IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_42.jpg)
![N/A IGFBP-3 [HRP]](https://images.novusbio.com/images2/DATA_IGFBP3_DGB300_ELISA_694.jpg)
![N/A IGFBP-3 [HRP]](https://images.novusbio.com/images2/IGFBP-3_DGB300_ELISA_104.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![N/A IGFBP-1 [Biotin]](https://images.novusbio.com/images2/DATA_IGFBP1_DY871_ELISA_2250.jpg)
![Western Blot IGFBP-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/IGFBP2_AF674_Western_Blot_20670.jpg)
![Simple Western IGFBP-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/IGFBP2_AF674_Simple_Western_20942.jpg)
![Neutralization IGFBP-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/IGFBP-2_AF674_Block_Neutralize_8943.jpg)
![Western Blot Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_Western_Blot_20961.jpg)
![ELISA Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_ELISA_22201.jpg)
![Neutralization Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_Block_Neutralize_8751.jpg)
![SDS-PAGE EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236-EG_234.jpg)
![Bioactivity EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236EG_1570.jpg)
![Cell Culture EGF [Unconjugated]](https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")