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IGF-I R/IGF1R Antibody (1H7)

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Flow Cytometry: IGF-I R Antibody (1H7) [NB100-66537] - Staining of human peripheral blood granulocytes with mouse anti human CD221 (NB100-66537).
Flow Cytometry: IGF-I R Antibody (1H7) [NB100-66537] - Staining of human peripheral blood granulocytes with mouse anti human CD221 (NB100-66537).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
1H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-66537AF350
Alexa Fluor 405
NB100-66537AF405
Alexa Fluor 488
NB100-66537AF488
Alexa Fluor 532
NB100-66537AF532
Alexa Fluor 594
NB100-66537AF594
Alexa Fluor 647
NB100-66537AF647
Alexa Fluor 700
NB100-66537AF700
Alexa Fluor 750
NB100-66537AF750
Allophycocyanin
NB100-66537APC
Allophycocyanin/Cy7
NB100-66537APCCY7
Biotin
NB100-66537B
CoraFluor 1
NB100-66537CL1
DyLight 350
NB100-66537UV
DyLight 405
NB100-66537V
DyLight 488
NB100-66537G
DyLight 550
NB100-66537R
DyLight 594
NB100-66537DL594
DyLight 650
NB100-66537C
DyLight 680
NB100-66537FR
DyLight 755
NB100-66537IR
FITC
NB100-66537F
HRP
NB100-66537H
Janelia Fluor 525
NB100-66537JF525
Janelia Fluor 549
NB100-66537JF549
Janelia Fluor 585
NB100-66537JF585
Janelia Fluor 635
NB100-66537JF635
Janelia Fluor 646
NB100-66537JF646
Janelia Fluor 669
NB100-66537JF669
PE
NB100-66537PE
PE/Atto594
NB100-66537PEATT594
PE/Cy5.5
NB100-66537PECY55
PE/Cy7
NB100-66537PECY7
PerCP
NB100-66537PCP
mFluor Violet 450 SE
NB100-66537MFV450
mFluor Violet 500 SE
NB100-66537MFV500
mFluor Violet 610 SE
NB100-66537MFV610
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IGF-I R/IGF1R Antibody (1H7) Summary

Immunogen
Purified human placental IGF-I receptor
Specificity
NB100-66537 recognizes human CD221
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGF1R
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:40
  • Western Blot 1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. This product requires protein digestion pretreatment of paraffin sections e.g. trypsin or pronase.
Control
IGF-I R/IGF1R Overexpression Lysate

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IGF-I R/IGF1R Antibody (1H7)

  • CD221 antigen
  • CD221
  • EC 2.7.10
  • EC 2.7.10.1
  • IGF1R
  • IGF-1R
  • IGF-I R
  • IGF-I receptor
  • IGFIR
  • IGF-IR
  • IGFR
  • insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • JTK13
  • MGC142170
  • MGC142172
  • MGC18216
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2

Background

There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry EGFR Antibody [Unconjugated]
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DGB300
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AF1230
Western Blot ERK2 Antibody [Unconjugated]
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Knockout Validated ERK2 Antibody [Unconjugated]
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Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
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Antibody [Unconjugate
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Antibody [Unconjugate
AF1067
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(15 Publications)
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DY871
N/A IGFBP-1 [Biotin]
(16 Publications)
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236-EG
SDS-PAGE EGF [Unconjugated]
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(793 Publications)
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Antibody
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NB300-514
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(12 Publications)
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Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
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AF578
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Western Blot IGFBP-5 Antibody
Western Blot IGFBP-5 Antibody
(7 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB

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Control Lysate(s)

IGF-I R/IGF1R Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IGF1R
Entrez
Uniprot