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Monkey IgE ELISA Kit (Colorimetric)

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ELISA: Monkey IgE ELISA Kit (Colorimetric) [NBP3-23448] - Samples were spiked with high concentrations of Monkey IgE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Monkey IgE ELISA Kit (Colorimetric) [NBP3-23448] - Standard Curve Reference

Product Details

Summary
Reactivity PmSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.50 - 4000 pg/mL

Order Details

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Monkey IgE ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Monkey IgE in samples. No significant cross-reactivity or interference between Monkey IgE and analogues was observed.
Standard Curve Range
62.50 - 4000 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 6.49
Intra-Assay
%CV < 5.66
Spike Recovery
84 - 109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IGHE

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Monkey IgE ELISA Kit (Colorimetric)

  • IgE
  • immunoglobulin heavy constant epsilon

Background

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Blogs on IgE.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHE