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IFN-gamma Antibody (K3.53) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA, ELISA(Cap), ELISA(Det), Flow
Clone
K3.53
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2852U-100UG
Alexa Fluor 405
IC2852V-100UG
Alexa Fluor 488
IC2852G-100UG
Alexa Fluor 532
FAB2852X
Alexa Fluor 594
IC2852T-100UG
Alexa Fluor 647
IC2852R-100UG
Alexa Fluor 700
IC2852N-100UG
Alexa Fluor 750
IC2852S-100UG
Allophycocyanin
FAB2852A
Biotin
FAB2852B
DyLight 350
FAB2852D
DyLight 405
FAB2852E
DyLight 488
FAB2852K
DyLight 550
FAB2852L
DyLight 594
FAB2852M
DyLight 650
FAB2852W
DyLight 680
FAB2852Y
DyLight 755
FAB2852Z
FITC
FAB2852F
HRP
FAB2852H
Janelia Fluor 525
FAB2852JF525
Janelia Fluor 549
FAB2852I
Janelia Fluor 585
FAB2852JF585
Janelia Fluor 635
FAB2852JF635
Janelia Fluor 646
FAB2852J
Janelia Fluor 669
FAB2852JF669
PE
FAB2852P
PerCP
FAB2852C
Unconjugated
MAB2852-100
mFluor Violet 450 SE
FAB2852MFV450
mFluor Violet 500 SE
FAB2852MFV500
mFluor Violet 610 SE
FAB2852MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2852-100
Carrier Free
MAB2852-SP
Carrier Free
MAB2852-500
Carrier Free
MAB2852-100
Carrier Free
IC2852V-100UG
Carrier Free
IC2852U-100UG
Carrier Free
IC2852T-100UG
Carrier Free
IC2852S-100UG
Carrier Free
IC2852R-100UG
Carrier Free
IC2852N-100UG
Carrier Free
IC2852G-100UG
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IFN-gamma Antibody (K3.53) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
E. coli-derived recombinant human IFN-gamma
Specificity
Detects human IFN‑ gamma in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse IFN‑ gamma , recombinant rat IFN‑ gamma , recombinant porcine IFN‑ gamma , recombinant feline IFN‑ gamma , or recombinant cotton rat IFN‑ gamma is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IFNG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry
  • Neutralization
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for IFN-gamma Antibody (K3.53) [CoraFluor™ 1]

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IFN-gamma Antibody
(K3.53) [CoraFluor ...
FAB2852CL1
IFN-gamma Antibody (K3.53) [CoraFluor™ 1]
Species: Hu
Applications: WB, B/N, ELISA, ELISA(Cap), ELISA(Det), Flow

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IFN-gamma Antibody (FAB2852CL1). (Showing 1 - 1 of 1 FAQ).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IFNG