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ICAM-3/CD50 Antibody (OTI1E7)

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Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM-3.
Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM-3.
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of extracts (35ug) from 9 different cell lines by using ICAM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ICAM3.

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70975AF350
Alexa Fluor 405
NBP2-70975AF405
Alexa Fluor 488
NBP2-70975AF488
Alexa Fluor 532
NBP2-70975AF532
Alexa Fluor 594
NBP2-70975AF594
Alexa Fluor 647
NBP2-70975AF647
Alexa Fluor 700
NBP2-70975AF700
Alexa Fluor 750
NBP2-70975AF750
Allophycocyanin
NBP2-70975APC
Biotin
NBP2-70975B
CoraFluor 1
NBP2-70975CL1
DyLight 350
NBP2-70975UV
DyLight 405
NBP2-70975V
DyLight 488
NBP2-70975G
DyLight 550
NBP2-70975R
DyLight 594
NBP2-70975DL594
DyLight 650
NBP2-70975C
DyLight 680
NBP2-70975FR
DyLight 755
NBP2-70975IR
FITC
NBP2-70975F
HRP
NBP2-70975H
Janelia Fluor 525
NBP2-70975JF525
Janelia Fluor 549
NBP2-70975JF549
Janelia Fluor 585
NBP2-70975JF585
Janelia Fluor 635
NBP2-70975JF635
Janelia Fluor 646
NBP2-70975JF646
Janelia Fluor 669
NBP2-70975JF669
PE
NBP2-70975PE
PerCP
NBP2-70975PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70975
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ICAM-3/CD50 Antibody (OTI1E7) Summary

Immunogen
Full length human recombinant protein of human ICAM3(NP_002153) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ICAM3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:400-4000
Theoretical MW
56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ICAM-3/CD50 Antibody (OTI1E7)

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Background

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ICAM3