Home » DC-SIGNR/CD299/CLEC4M » DC-SIGNR/CD299/CLEC4M Antibodies » DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]
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DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]

 (18 Publications)
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Human DC‑SIGNR/CD299 and DC‑SIGN transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGNR/CD299 Monoclonal Antibody (Catalog # MAB162, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Human DC‑SIGNR/CD299 and DC‑SIGN transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGNR/CD299 Monoclonal Antibody (Catalog # MAB162, filled histograms) or isotype control ...read more
In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. ...read more
In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
120604
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB162U-100UG
Alexa Fluor 405
FAB162V-100UG
Alexa Fluor 488
FAB162G-100UG
Alexa Fluor 532
FAB162X
Alexa Fluor 594
FAB162T-100UG
Alexa Fluor 647
FAB162R-100UG
Alexa Fluor 700
FAB162N-100UG
Alexa Fluor 750
FAB162S-100UG
Allophycocyanin
FAB162A
Allophycocyanin/Cy7
FAB162APCCY7
Biotin
FAB162B
DyLight 350
FAB162D
DyLight 405
FAB162E
DyLight 488
FAB162K
DyLight 550
FAB162L
DyLight 594
FAB162M
DyLight 650
FAB162W
DyLight 680
FAB162Y
DyLight 755
FAB162Z
HRP
FAB162H
Janelia Fluor 525
FAB162JF525
Janelia Fluor 549
FAB162I
Janelia Fluor 585
FAB162JF585
Janelia Fluor 635
FAB162JF635
Janelia Fluor 646
FAB162J
Janelia Fluor 669
FAB162JF669
PE/Atto594
FAB162PEATT594
PE/Cy5.5
FAB162PECY55
PE/Cy7
FAB162PECY7
PerCP
FAB162C
Phycoerythrin
FAB162P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB162P
Carrier Free
MAB162-SP
Carrier Free
FAB162V-100UG
Carrier Free
FAB162U-100UG
Carrier Free
FAB162T-100UG
Carrier Free
FAB162S-100UG
Carrier Free
FAB162R-100UG
Carrier Free
FAB162N-100UG
Carrier Free
FAB162G-100UG
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DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated] Summary

Additional Information
Clone 120604 was used by HLDA to establish CD designation
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3
Specificity
Detects human DC‑SIGNR/CD299.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLEC4M
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Adhesion Blockade 2.5 ug/mL
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB162 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]

  • CD209L
  • CD209L1
  • CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2
  • CD299 antigenMGC129964
  • CD299
  • CLEC4M
  • C-type lectin domain family 4, member M
  • DC-SIGN2
  • DC-SIGN2CD209 antigen-like protein 1
  • DCSIGNR
  • DC-SIGNR
  • DC-SIGNRDC-SIGN-related protein
  • DCSIGNRMGC47866
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 2
  • HP10347
  • liver/lymph node-specific ICAM-3 grabbing non-integrin
  • Liver/lymph node-specific ICAM-3-grabbing non-integrin
  • LSIGN
  • L-SIGN
  • LSIGNC-type lectin domain family 4 member M
  • mannose binding C-type lectin DC-SIGNR

Background

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

  1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
  3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
  4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  5. Feinberg, H. et al. (2001) Science 294:2163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DC-SIGNR/CD299/CLEC4M Antibody (MAB162)(18)

We have publications tested in 2 confirmed species: Human, Primate - Cercopithecus aethiops (African Green Monkey).

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization.


Filter By Application
Flow Cytometry
(5)
ICC
(1)
IHC
(2)
IHC-Fr
(1)
Neutralization
(4)
All Applications
Filter By Species
Human
(13)
Primate - Cercopithecus aethiops (African Green Monkey)
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using MAB162 Applications Species
Shimojima, M;Sugimoto, S;Taniguchi, S;Maeki, T;Yoshikawa, T;Kurosu, T;Tajima, S;Lim, CK;Ebihara, H; N-glycosylation of viral glycoprotein is a novel determinant for the tropism and virulence of highly pathogenic tick-borne bunyaviruses PLoS pathogens 2024-07-15 [PMID: 39008518] (Flow Cytometry, Human, Primate - Cercopithecus aethiops (African Green Monkey)) Flow Cytometry Human, Primate - Cercopithecus aethiops (African Green Monkey)
Lei Guo, Yan Liang, Heng Li, Huiwen Zheng, Zening Yang, Yanli Chen et al. Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors iScience 12/17/2021 [PMID: 34786539]
AW Wessel, MP Doyle, TB Engdahl, J Rodriguez, JE Crowe, MS Diamond Human Monoclonal Antibodies against NS1 Protein Protect against Lethal West Nile Virus Infection MBio, 2021-10-12;12(5):e0244021. 2021-10-12 [PMID: 34634945] (Human) Human
Y Kondo, JL Larabee, L Gao, H Shi, B Shao, CM Hoover, JM McDaniel, YC Ho, R Silasi-Man, SA Archer-Har, P Azadi, RS Srinivasan, AR Rezaie, A Borczuk, JC Laurence, F Lupu, J Ahamed, RP McEver, JF Papin, Z Yu, L Xia L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus JCI Insight, 2021-07-22;6(14):. 2021-07-22 [PMID: 34291736] (Flow Cytometry, Neutralization, Human) Flow Cytometry, Neutralization Human
Menglan Xiang, Rubén Adrián Grosso, Akira Takeda, Junliang Pan, Tove Bekkhus, Kevin Brulois et al. A Single-Cell Transcriptional Roadmap of the Mouse and Human Lymph Node Lymphatic Vasculature Frontiers in Cardiovascular Medicine 4/30/2020 [PMID: 32426372]
MB Vogt, A Lahon, RP Arya, JL Spencer Cl, R Rico-Hesse Dengue viruses infect human megakaryocytes, with probable clinical consequences PLoS Negl Trop Dis, 2019-11-25;13(11):e0007837. 2019-11-25 [PMID: 31765380] (Flow Cytometry, Human) Flow Cytometry Human
Derek L. Carbaugh, Ralph S. Baric, Helen M. Lazear Envelope Protein Glycosylation Mediates Zika Virus Pathogenesis Journal of Virology 6/15/2019 [PMID: 30944176]
Goo L, Debbink K, Kose N et al. A protective human monoclonal antibody targeting the West Nile virus E protein preferentially recognizes mature virions. Nat Microbiol. 11/19/2018 [PMID: 30455471]
LL Swystun, JD Lai, C Notley, I Georgescu, AS Paine, J Mewburn, K Nesbitt, K Schledzews, C Géraud, J Kzhyshkows, S Goerdt, W Hopman, RR Montgomery, PD James, D Lillicrap The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity J. Clin. Invest., 2018-08-20;128(9):4057-4073. 2018-08-20 [PMID: 30124466] (Flow Cytometry, Human) Flow Cytometry Human
Mariko Ishibashi, Naoko Morita, Chisato Nomura-Kawaguchi, Yohko Shimizu, Takaji Wakita, Mariko Esumi CLEC4M-positive and CD81-negative Huh7 cells are not susceptible to JFH-1 HCVcc infection but mediate transinfection Archives of Virology 11/1/2014 [PMID: 24965233]
Show All 18 Publications.

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Bioinformatics

Gene Symbol CLEC4M
Uniprot