![Western Blot: iASPP Antibody [NB100-88155] - Detection of Human and Mouse iASPP by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), HeLa (50, 15 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-iASPP antibody NB100-88155 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/iASPP-Antibody-Western-Blot-NB100-88155-img0005.jpg "Western Blot: iASPP Antibody [NB100-88155] - Detection of Human and Mouse iASPP by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), HeLa (50, 15 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-iASPP antibody NB100-88155 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human inhibitor of apoptosis stimulating protein of p53 using the numbering given in entry NP_006654.2 (GeneID 10848). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PPP1R13L |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: iASPP Antibody [NB100-88155] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiiASPP antibody used for IP at 6 ug per reaction. iASPP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated iASPP, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes](http://images.novusbio.com/fullsize/iASPP-Antibody-Immunoprecipitation-NB100-88155-img0004.jpg "Immunoprecipitation: iASPP Antibody [NB100-88155] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiiASPP antibody used for IP at 6 ug per reaction. iASPP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated iASPP, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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