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53BP2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: 53BP2 Antibody [NBP1-90324] - Staining of human cell line A-431 shows localization to cytosol & cell junctions.
Immunohistochemistry: 53BP2 Antibody [NBP1-90324] - Staining of human testis shows strong nuclear positivity in cells of the seminiferus ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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53BP2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PQPSKDTLLPPFRKPQTVAASSIYSMYTQQQAPGKNFQQAVQSALTKTHTRGPHFSSVYGKPVIAAAQNQQQHPENIYSNSQGKPGSPEPETEPVSSVQENHENERI
Predicted Species
Mouse (91%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53BP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
53BP2 Protein (NBP1-90324PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for 53BP2 Antibody

  • apoptosis-stimulating protein of p53, 2
  • ASPP2P53BP2,53BP2apoptosis-stimulating of p53 protein 2
  • Bbp
  • Bcl2-binding protein
  • p53-binding protein 2
  • p53BP2
  • PPP1R13A
  • Renal carcinoma antigen NY-REN-51
  • tumor protein p53 binding protein, 2
  • tumor protein p53-binding protein, 2
  • Tumor suppressor p53-binding protein 2

Background

53BP2 encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TP53BP2