Novus Biologicals products are now on bio-techne.com

Human FGF basic/FGF2/bFGF Quantikine ELISA Kit

Images

 

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Human FGF basic/FGF2/bFGF Quantikine ELISA Kit Summary

Background
The Quantikine Human FGF basic Immunoassay kit is a 4.5 hour solid phase ELISA designed to measure human FGF basic in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FG...F basic showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.
Show More
Specificity
Natural and recombinant human FGF basic.  This assay cross-reacts 40% with natural Bovine FGF-basic, 0.15% with natural Bovine FGF-acidic, 17% with recombinant Bovine FGF-basic, 18% with recombinant Mouse FGF-basic and 57% with recombinant Rat FGF-basic.
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
FGF2

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DFB50.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human FGF basic/FGF2/bFGF Quantikine ELISA Kit

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • heparin-binding growth factor 2
  • Prostatropin

Background

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Customers Who Viewed This Item Also Viewed...

DVE00
Species: Hu
Applications: ELISA
236-EG
Species: Hu
Applications: BA
AF232
Species: Hu
Applications: IHC, Neut, Simple Western, WB
291-G1
Species: Hu
Applications: BA
NB600-1287
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
H00002263-M01
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
M6000B
Species: Mu
Applications: ELISA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
294-HG
Species: Hu
Applications: BA
DBD00
Species: Hu
Applications: ELISA
251-KG
Species: Hu
Applications: BA
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
267-N3
Species: Hu
Applications: BA
256-GF
Species: Hu
Applications: BA
DFB50
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for FGF basic/FGF2/bFGF (DFB50)(114)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Bovine, Canine, Ovine, Porcine, Rabbit, Tree Shrew.


Filter By Application
All Applications
Filter By Species
Human
(89)
Mouse
(7)
Rat
(7)
Bovine
(3)
Canine
(1)
Ovine
(1)
Porcine
(3)
Rabbit
(1)
Tree Shrew
(1)
All Species
Showing Publications 1 - 10 of 114. Show All 114 Publications.
Publications using DFB50 Applications Species
Yang, J;Lewis, GP;Hsiang, CH;Menges, S;Luna, G;Cho, W;Turovets, N;Fisher, SK;Klassen, H; Amelioration of Photoreceptor Degeneration by Intravitreal Transplantation of Retinal Progenitor Cells in Rats International journal of molecular sciences 2024-07-24 [PMID: 39125629] (Human) Human
Zhang, J;Li, J;Qu, X;Liu, Y;Sun, L;Harada, A;Hua, Y;Sougawa, N;Tabata, A;Liu, L;Miyagawa, S; Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction Bioactive materials 2024-07-01 [PMID: 38689657] (Human) Human
Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A; A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants Microorganisms 2024-04-02 [PMID: 38674664] (Human) Human
Ríos, S;González, LG;Saez, CG;Smith, PC;Escobar, LM;Martínez, CE; L-PRF Secretome from Both Smokers/Nonsmokers Stimulates Angiogenesis and Osteoblast Differentiation In Vitro Biomedicines 2024-04-16 [PMID: 38672228] (Human) Human
Pal, A;Oyane, A;Nakamura, M;Koga, K;Nishida, E;Miyaji, H; Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth Factor International journal of molecular sciences 2024-01-25 [PMID: 38338772] (Human) Human
Schönborn, M;Gregorczyk-Maga, I;Batko, K;Maga, M;Bogucka, K;Gawlik, K;Pawlica-Gosiewska, D;Maga, P; Angiogenic and Microvascular Status Alterations after Endovascular Revascularization of Lower Limb Arteries among Patients with Diabetic Foot Syndrome: A Prospective 12-Month Follow-Up Study Journal of clinical medicine 2023-08-27 [PMID: 37685648] (Human) Human
Yanagihara, M;Matsuno, Y;Ueno, K;Kurazumi, H;Suzuki, R;Tanaka, T;Hamano, K; Fibroblasts are the most suitable cell source for regenerative medicine due to their high intracellular fibroblast growth factor 2 content Biochemistry and biophysics reports 2023-09-01 [PMID: 37457362] (Human) Human
Amini, M;Venkatesan, JK;Nguyen, TN;Liu, W;Leroux, A;Madry, H;Migonney, V;Cucchiarini, M; rAAV TGF-beta and FGF-2 Overexpression via pNaSS-Grafted PCL Films Stimulates the Reparative Activities of Human ACL Fibroblasts International journal of molecular sciences 2023-07-06 [PMID: 37446318] (Human) Human
Schönborn, M;Gregorczyk-Maga, I;Batko, K;Bogucka, K;Maga, M;P?otek, A;Pasieka, P;S?owi?ska-Solnica, K;Maga, P; Circulating Angiogenic Factors and Ischemic Diabetic Foot Syndrome Advancement-A Pilot Study Biomedicines 2023-05-27 [PMID: 37371653] (Human) Human
LV Trevor, K Riches-Sum, AL Mahajan, MJ Thornton Stromal Vascular Fraction Cells from Individuals Who Have Previously Undergone Radiotherapy Retain Their Pro-Wound Healing Properties Journal of Clinical Medicine, 2023-03-04;12(5):. 2023-03-04 [PMID: 36902839] (Human) Human
Show All 114 Publications.

Reviews for FGF basic/FGF2/bFGF (DFB50) (0)

There are no reviews for FGF basic/FGF2/bFGF (DFB50). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FGF basic/FGF2/bFGF (DFB50). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Additional FGF basic/FGF2/bFGF Products

Blogs on FGF basic/FGF2/bFGF

There are no specific blogs for FGF basic/FGF2/bFGF, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human FGF basic/FGF2/bFGF Quantikine ELISA Kit and receive a gift card or discount.

Bioinformatics

Gene Symbol FGF2