I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: Recombinant Human FGF basic/FGF2/bFGF Protein
Brand: Novus Biologicals
SKU: NBP2-34921
Rating: 5 (2 reviews)

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Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our  NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	PAGE, Bioactivity
Format	Carrier-Free
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier-Free NBP2-34921-1mg

Carrier-Free NBP2-34921-100ug

Carrier-Free NBP2-34921-500ug

Description	A single non-glycosylated polypeptide chain containing 147 amino acids corresponding to FGF basic/FGF2/bFGF Source: E. coli Uniprot ID: P09038 Amino Acid Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Details of Functionality	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg.
Source	E. coli
Protein/Peptide Type	Recombinant Protein
Gene	FGF2
Purity	>95%, by SDS-PAGE and HPLC
Endotoxin Note	Less than 1 EU/ug of FGF basic/FGF2/bFGF as determined by LAL method.

Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, 150 mM NaCl, 0.02% Tween-20, pH 7.6.
Preservative	5% Trehalose
Concentration	LYOPH
Purity	>95%, by SDS-PAGE and HPLC
Reconstitution Instructions	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Recombinant Human FGF basic/FGF2/bFGF Protein

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Publications using NBP2-34921	Applications	Species
Lovric A, Rassolie A, Alam S et al. Single-cell sequencing deconvolutes cellular responses to exercise in human skeletal muscle Communications biology 2022-10-22 [PMID: 36273106]
Abdul M, IbaNez D, Zhao P et al. Generation of an induced pluripotent stem cell line (GIBHi003-A) from a Parkinson's disease patient with mutant PINK1 (p. I368N) Stem Cell Research 2019-10-01 [PMID: 31778937]
Nikolaou S, Hadjikypri X, Ioannou G et al. Functional and phenotypic distinction of the first two trophoblast subdivisions and identification of the border between them during early postimplantation: A prerequisite for understanding early patterning during placentogenesis. Biochem Biophys Res Commun 2018-01-29 [PMID: 29305264]
Li X, Wang Z, Jiang Z et al. Regulation of seminiferous tubule-associated stem Leydig cells in adult rat testes. Proc. Natl. Acad. Sci. U.S.A. 2016-03-08 [PMID: 26929346]
Chao Feng, Jie Li, Jinlan Ruan, Kan Ding. MicroRNA-125a inhibits cell growth by targeting glypican-4. Glycoconj J 2012-10-01 [PMID: 22644326]

Li Y, IbaNez D, Fan W et al. Generation of an induced pluripotent stem cell line (GIBHi004-A) from a Parkinson's disease patient with mutant DJ-1/PARK7 (p.L10P) Stem Cell Research 2020-05-01 [PMID: 32534165] (Human)		Human

	Verified Customer 01/04/2017
Application:	SDS-PAGE Control
Species:

Gene Symbol	FGF2
Uniprot	P09038() P09038() P09038()