| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Conjugate | HRP |
| Background | The Quantikine Human Cyr61/CCN1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cyr61 in cell culture supernates, serum, plasma, saliva, and human milk. It contains CHO cell-expressed recombinant human Cyr61 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cyr61 showed linear curves that were parallel to the standa...rd curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Cyr61. Show More |
| Specificity | Natural and recombinant human Cyr61 |
| Source | N/A |
| Assay Type | Solid Phase Sandwich ELISA |
| Inter-Assay | See PDF Datasheet for details |
| Intra-Assay | See PDF Datasheet for details |
| Spike Recovery | See PDF Datasheet for details |
| Sample Volume | See PDF Datasheet for details |
| Gene | CYR61 |
| Dilutions |
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| Application Notes | No significant interference observed with available related molecules. |
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| Publications |
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| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa matricellular glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis through interactions with integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration.
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| Gene Symbol | CYR61 |
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![Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Sections of the ectopic lesions collected from E2 or E2 plus P4-treated recipients (D16, n = 6) were subjected to histological examination. (B) Representative images (20X) showing IHC analysis using antibodies against an angiogenetic regulator CCN1. The immunostaining intensity of CCN1 was analyzed by ImageJ software. The numerical values were analyzed by One-way ANOVA followed by Dunnett's post hoc test and expressed as mean +/- SEM. Statistical significance is defined as *: p<0.01. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0165347) licensed under a CC-BY license.](https://images.novusbio.com/images/Cyr61-CCN1-Antibody-Immunohistochemistry-NB100-356-img0007.jpg "Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Sections of the ectopic lesions collected from E2 or E2 plus P4-treated recipients (D16, n = 6) were subjected to histological examination. (B) Representative images (20X) showing IHC analysis using antibodies against an angiogenetic regulator CCN1. The immunostaining intensity of CCN1 was analyzed by ImageJ software. The numerical values were analyzed by One-way ANOVA followed by Dunnett's post hoc test and expressed as mean +/- SEM. Statistical significance is defined as *: p<0.01. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0165347) licensed under a CC-BY license.")