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Human/Canine/Porcine Insulin DuoSet ELISA, 5 Plate

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Human/Canine/Porcine Insulin DuoSet ELISA, 5 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
INS

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY8056-05.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Canine/Porcine Insulin DuoSet ELISA, 5 Plate

  • IDDM2
  • ILPR
  • INS
  • Insulin
  • IRDN
  • MODY10
  • proinsulin

Background

Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes.

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Publications for Insulin (DY8056-05)(7)

We have publications tested in 1 confirmed species: Human.


Filter By Application
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using DY8056-05 Applications Species
Cherpaz, M;Meugnier, E;Seillier, G;Pozzi, M;Pierrard, R;Leboube, S;Farhat, F;Vola, M;Obadia, JF;Amaz, C;Chalabreysse, L;May, C;Chanon, S;Brun, C;Givre, L;Bidaux, G;Mewton, N;Derumeaux, G;Bergerot, C;Paillard, M;Thibault, H; Myocardial transcriptomic analysis of diabetic patients with aortic stenosis: key role for mitochondrial calcium signaling Cardiovascular diabetology 2024-07-08 [PMID: 38978010] (Human) Human
A Dall'Agnes, JM Platt, MM Zheng, M Friesen, G Dall'Agnes, AM Blaise, JB Spinelli, JE Henninger, EN Tevonian, NM Hannett, C Lazaris, HK Drescher, LM Bartsch, HR Kilgore, R Jaenisch, LG Griffith, II Cisse, JF Jeppesen, TI Lee, RA Young The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance Nature Communications, 2022-12-06;13(1):7522. 2022-12-06 [PMID: 36473871] (Human) Human
V Mocanu, Z Zhang, EC Deehan, DH Kao, N Hotte, S Karmali, DW Birch, KK Samarasing, J Walter, KL Madsen Fecal microbial transplantation and fiber supplementation in patients with severe obesity and metabolic syndrome: a randomized double-blind, placebo-controlled phase 2 trial Nature Medicine, 2021-07-05;27(7):1272-1279. 2021-07-05 [PMID: 34226737] (Human) Human
LI Lyngfelt, MC Erlandsson, M Nadali, S Hedjazifar, R Pullerits, KM Andersson, P Brembeck, ST Silfverswä, U Smith, MI Bokarewa Impact of the Uncoupling Protein 1 on Cardiovascular Risk in Patients with Rheumatoid Arthritis Cells, 2021-05-07;10(5):. 2021-05-07 [PMID: 34067093] (Human) Human
LWM Loo, K Nishibun, L Welsh, T Makolo, CD Chong, I Pagano, H Yu, EO Bantum Using a cultural dance program to increase sustainable physical activity for breast cancer survivors-A pilot study Complement Ther Med, 2019-09-21;47(0):102197. 2019-09-21 [PMID: 31780003] (Human) Human
MC Erlandsson, L Lyngfelt, ND Åberg, C Wasén, RA Espino, ST Silfverswä, M Nadali, K Jood, KME Andersson, R Pullerits, MI Bokarewa Low serum IGF1 is associated with hypertension and predicts early cardiovascular events in women with rheumatoid arthritis BMC Med, 2019-07-22;17(1):141. 2019-07-22 [PMID: 31327319] (Human) Human
A Binia, C Vargas-Mar, M Ancira-Mor, LM Gosoniu, I Montoliu, E Gámez-Vald, DC Soria-Cont, A Angeles-Qu, R Gonzalez-A, S Fernández, D Martínez-C, B Hernández-, M Ramírez-So, C Pérez-Orte, Y Rodríguez-, I Castan, I Rubio-Alia, F Vadillo-Or, R Márquez-Ve, R Bojalil, JC López-Alva, P Valet, M Kussmann, I Silva-Zole, ME Tejero Improvement of cardiometabolic markers after fish oil intervention in young Mexican adults and the role of PPAR? L162V and PPAR?2 P12A J. Nutr. Biochem., 2017-02-10;43(0):98-106. 2017-02-10 [PMID: 28282585] (Human) Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Insulin (DY8056-05). (Showing 1 - 1 of 1 FAQs).

  1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
    • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

Additional Insulin Products

Blogs on Insulin. Showing 1-10 of 13 blog posts - Show all blog posts.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.

Insulin signaling in adipocytes: Carbohydrate-signaling transcription factor ChREBP is the link between lipolytic enzyme Hormone-Sensitive Lipase and lipogenic enzyme ELOVL6
By Jamshed Arslan, Pharm. D., PhD. Insulin resistance in adipocytes is a major feature of metabolic syndrome   . Disrupted adipose tissue metabolism can lead to accumulation of lipid intermediates in insul...  Read full blog post.

Inhibiting incretin GIP hormone activity in mouse and monkey models to combat obesity
By Jamshed Arslan, Pharm. D., PhD. We live in a world where 39% of adults are overweight. Our meals trigger the secretion of various gut-derived metabolic hormones called incretins. Fats and carbohydrates in our die...  Read full blog post.

Insulin signaling in brain’s subfornical organ is crucial for regulating cardiometabolic profile
By Jamshed Arslan, Pharm. D., PhD. The hypothalamus is an insulin receptor-rich brain region. Insulin receptor signaling in the CNS can regulate blood pressure, for example, by increasing sympathetic outflow to th...  Read full blog post.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in ...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol INS