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hnRNP L Antibody (4D11) - Azide and BSA Free

Images

 
Western Blot: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778] - Whole cell protein from HeLa (lane 1), A431 (lane 2), NIH-3T3 (lane 3) and PC12 (lane 4) was separated by SDS-PAGE and transferred to PVDF ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778] - hnRNP L antibody was tested in HeLa cells at 5 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin ...read more
Immunohistochemistry: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778] - Immunohistochemistry-Frozen: hnRNP L Antibody (4D11) [NB120-6106] Image from the standard format of this antibody.
Western Blot: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778] - analysis of hnRNP L in HEK whole cell lysate using anti-hnRNP L antibody. Image from verified customer review. Image from the standard format of ...read more
Western Blot: hnRNP L Antibody (4D11) - Azide and BSA Free [NBP2-80778] - Western blot for anti-hnRNP-L on HeLa cell extracts Image from the standard format of this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Bv, Ch, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clone
4D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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hnRNP L Antibody (4D11) - Azide and BSA Free Summary

Immunogen
Human hnRNP proteins from HeLa cells purified by affinity chromatography on ssDNA agarose.
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPL
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:5000

Reactivity Notes

Newt.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for hnRNP L Antibody (4D11) - Azide and BSA Free

  • FLJ35509
  • heterogeneous nuclear ribonucleoprotein L
  • hnRNP L
  • hnRNP-L
  • HNRPL

Background

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (HNRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with HNRNP complexes and along with other HNRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of HNRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Strucutrally, hnRPL contains 2 segments of approximately 80 amino acids each which are weakly related to each other and to the ribonucleoprotein consensus sequence-type RNA-binding domains of other HNRNP and snRNP proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HNRNPL