hnRNP A1 Antibody (4B10) - BSA Free

RNA polymerase II trans cripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, premRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts.2, 4, 5 Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa.2, 4, 5 hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein binds to its RNA target through a consensus RNA-binding site UAGGGU. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP -A1 from transportin. hnRNP-A1 is ubiquitously expressed with a higher expression in proliferating and/or transformed cells than in differentiated tissues.6

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA, ICC/IF, IP, WB.

Showing Publications 1 - 10 of 23. Show All 23 Publications. Collapse Publications.

Publications using NB100-672	Applications	Species
Huelga SC, Vu AQ, Arnold JD et al. Integrative genome-wide analysis reveals cooperative regulation of alternative splicing by hnRNP proteins Cell Rep 2012-02-01 [PMID: 22574288] (Human)		Human
Bonnal S, Pileur F, Orsini C et al. Heterogeneous nuclear ribonucleoprotein A1 is a novel internal ribosome entry site trans-acting factor that modulates alternative initiation of translation of the fibroblast growth factor 2 mRNA. J Biol Chem. 2005-02-11 [PMID: 15525641] (WB, IP, Human)	WB, IP	Human
Pino I, Pio R, Toledo G et al. Altered patterns of expression of members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family in lung cancer. Lung Cancer. 2003-08-01 [PMID: 12871776] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Guil S, Gattoni R, Carrascal M et al. Roles of hnRNP A1, SR proteins, and p68 helicase in c-H-ras alternative splicing regulation. Mol Cell Biol. 2003-04-01 [PMID: 12665590] (WB, Human)	WB	Human
Mili S, Shu HJ, Zhao Y et al. Distinct RNP complexes of shuttling hnRNP proteins with pre-mRNA and mRNA: candidate intermediates in formation and export of mRNA. Mol Cell Biol. 2001-11-01 [PMID: 11585913] (WB, Human)	WB	Human
van der Houven van Oordt W, Diaz-Meco MT, Lozano J et al. The MKK(3/6)-p38-signaling cascade alters the subcellular distribution of hnRNP A1 and modulates alternative splicing regulation. J Cell Biol. 2000-04-17 [PMID: 10769024] (ICC/IF, IP, WB, Mouse)	ICC/IF, IP, WB	Mouse
Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer. Mol Cell Biol. 1999-01-01 [PMID: 9858549] (IP, Human)	IP	Human
Siomi MC, Eder PS, Kataoka N et al. Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins. J Cell Biol. 1997-09-22 [PMID: 9298975] (WB, ICC/IF, IP, Human)	WB, ICC/IF, IP	Human
Pinol-Roma S, Choi YD, Matunis MJ, Dreyfuss G. Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins. Genes Dev. 1988-02-01 [PMID: 3129338] (IP, WB, ELISA, ICC/IF, Human)	IP, WB, ELISA, ICC/IF	Human
Pinol-Roma S, Swanson MS, Gall JG, Dreyfuss G. A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts. J Cell Biol. 1989-12-01 [PMID: 2687284] (ICC/IF, Human)	ICC/IF	Human
Pinol-Roma S, Dreyfuss G. Transcription-dependent and transcription-independent nuclear transport of hnRNP proteins. Science. 1991-07-19 [PMID: 1857966] (ICC/IF, Human)	ICC/IF	Human
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
Chen Y, Wu Y, Li J et al. Cooperative regulation of Zhx1 and hnRNPA1 drives the cardiac progenitor-specific transcriptional activation during cardiomyocyte differentiation Cell death discovery 2023-07-14 [PMID: 37452012] (WB, Mouse)	WB	Mouse
Fang MY, Markmiller S, Vu AQ, et al. Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD Neuron 2019-06-18 [PMID: 31272829]
Marrone, L;Drexler, HCA;Wang, J;Tripathi, P;Distler, T;Heisterkamp, P;Anderson, EN;Kour, S;Moraiti, A;Maharana, S;Bhatnagar, R;Belgard, TG;Tripathy, V;Kalmbach, N;Hosseinzadeh, Z;Crippa, V;Abo-Rady, M;Wegner, F;Poletti, A;Troost, D;Aronica, E;Busskamp, V; FUS pathology in ALS is linked to alterations in multiple ALS-associated proteins and rescued by drugs stimulating autophagy Acta Neuropathol. 2019-04-01 [PMID: 30937520] (ICC/IF, Human)	ICC/IF	Human
Thompson VF, Victor RA, Morera AA et al. Transcription-Dependent Formation of Nuclear Granules Containing FUS and RNA Pol II Biochemistry 2018-12-26 [PMID: 30488693]
Gasperini L, Rossi A, Cornella N et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol. Biol. Cell. 2018-10-24 [PMID: 30354839] (WB, Human)	WB	Human
Lampe S, Kunze M, Scholz A et al. Identification of the TXNIP IRES and characterization of the impact of regulatory IRES trans-acting factors. Biochim Biophys Acta Gene Regul Mech 2018-02-01 [PMID: 29378331] (WB)	WB
Hu J, Khodadadi-Jamayran A, Mao M et al. AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors. Nat Commun. 2016-11-08 [PMID: 27824034]
Kamelgarn M, Chen J, Kuang L et al. Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS Biochim. Biophys. Acta 2016-07-25 [PMID: 27460707] (WB)	WB
Kunze MM, Benz F, Braub TF et al. sST2 translation is regulated by FGF2 via an hnRNP A1-mediated IRES-dependent mechanism. Biochim. Biophys. Acta. 2016-05-08 [PMID: 27168114] (ICC/IF, WB, IP, Human)	ICC/IF, WB, IP	Human
Fujiya M, Konishi H, Mohamed Kamel MK et al. microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1 Oncogene 2013-10-28 [PMID: 24166503] (WB, Human) Details: This citation used the Alkaline Phosphatase version of this antibody.	WB	Human
Lin, Y-T, Yen, P H. A novel nucleocytoplasmic shuttling sequence of DAZAP1, a testis-abundant RNA-binding protein, . 12, 1486-1493. 2006-01-01 [PMID: 16772659]
Show All 23 Publications. Collapse Publications.

Array NB100-672

• hnRNP A1 Antibodies
• hnRNP A1 Lysates
• hnRNP A1 Proteins