I am looking for the recombinant human HMGB-1 which we would like to use for cultured human intervertebral disc cells in vitro. 1)Is your HMGB-1 protein NBC1-18488 already sterilized? 2)Are there some articles in which NBC1-18488 is used for human cultured cells in vitro?

Name: Recombinant Human HMGB1/HMG-1 His Protein
Brand: Novus Biologicals
SKU: NBC1-18488
Price: 429 USD

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Accepted Answer

NBC1-18488 is not sterilized, and may not be suitable for use in live cells. We have only approved this protein to work for ELISA and western blot, and have not assessed its biological activity. As such, we do not have any references that cite this product in cell culture.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	PAGE
Concentration	1 mg/ml

Description	A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-215 of Human HMGB1/HMG-1 Source: Baculovirus Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH
Source	Baculovirus
Protein/Peptide Type	Recombinant Protein
Gene	HMGB1
Purity	>90%, by SDS-PAGE
Endotoxin Note	< 1.0 EU per 1 microgram of protein (determined by LAL method)

Dilutions	SDS-Page
Theoretical MW	25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	20 mM Tris-HCl buffer (pH 8.0), 1 mM EDTA, 0.5 MM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Purity	>90%, by SDS-PAGE

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