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HMGB2 Antibody (8P10V5)

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Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of extracts of various cell lines, using HMGB2 Rabbit mAb (NBP3-16774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunofluorescence analysis of U-2 OS cells using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunohistochemistry of paraffin-embedded mouse testis using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Chromatin Immunoprecipitation: HMGB2 Antibody (8P10V5) [NBP3-16774] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using HMGB2 antibody (NBP3-16774) and rabbit IgG.The amount of immunoprecipitated ...read more
Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunofluorescence analysis of C6 cells using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunofluorescence analysis of NIH-3T3 cells using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774] - Rat testis using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 ...read more
Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774] - Human colon using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH ...read more
Immunoprecipitation: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of 300ug extracts of Mouse testis cells using 3ug HMGB2 antibody (NBP3-16774). Western blot was performed from the immunoprecipitate using HMGB2 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP
Clone
8P10V5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HMGB2 Antibody (8P10V5) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGB2 (P26583). MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPS
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HMGB2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:500 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for HMGB2 Antibody (8P10V5)

  • High mobility group protein 2
  • high mobility group protein B2
  • high-mobility group box 2
  • HMG-2
  • HMG2high-mobility group (nonhistone chromosomal) protein 2

Background

HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HMGB2 Antibody (NBP3-16774) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HMGB2