Human HMGB1/HMG-1 ELISA Kit (Colorimetric) Summary
Specificity This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.
Standard Curve Range 31.25 - 2000 pg/mL
Sensitivity 18.75 pg/mL
Assay Type Sandwich-ELISA
Inter-Assay %CV < 4.52
Intra-Assay %CV < 5.09
Spike Recovery 93 - 108%
Sample Volume 100 uL
Kit Type ELISA Kit (Colorimetric)
Gene HMGB1
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Packaging, Storage & Formulations
Storage Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Biotinylated Detection Ab Diluent Concentrated Biotinylated Detection Ab (100x) Concentrated HRP Conjugate (100x) Concentrated Wash Buffer (25x) HRP Conjugate Diluent Micro ELISA Plate (Dismountable) Plate Sealer Product Manual Reference Standard Sample Diluent Stop Solution Substrate Reagent
Alternate Names for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)
Background
HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for HMGB1/HMG-1 ELISA Kit (NBP2-62766)(36)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 2 applications: ELISA, S-ELISA.
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Hall DA, Voigt RM, Cantu-Jungles TM et al. An open label, non-randomized study assessing a prebiotic fiber intervention in a small cohort of Parkinson's disease participants Nature communications 2023-02-18 [PMID: 36801916] (ELISA) ELISA
Furtado Milao JFI Utilizing cytotoxic lymphocytes for indirect shock-and-kill strategy in HIV-1 treatment Thesis 1905-07-13 (ELISA)
ELISA
Sunil V, Teoh JH, Mohan BC et al. Bioengineered immunomodulatory organelle targeted nanozymes for photodynamic immunometabolic therapy Journal of controlled release : official journal of the Controlled Release Society 2022-08-22 [PMID: 35987351]
Balsamo JA, Penton KE, Zhao Z et al. An immunogenic cell injury module for the single-cell multiplexed activity metabolomics platform to identify promising anti-cancer natural products The Journal of biological chemistry 2022-08-02 [PMID: 35931117] (ELISA, Human) ELISA
Human
Jang SB, Jin SM, Kim HS et al. DAMP-modulating nanoparticle for successful pancreatic islet and stem cell transplantation Biomaterials 2022-07-12 [PMID: 35849998] (ELISA) ELISA
Jarosova J, Macinga P, Krupickova L et al. Impact of Endoluminal Radiofrequency Ablation on Immunity in Pancreatic Cancer and Cholangiocarcinoma Biomedicines 2022-06-06 [PMID: 35740353] (ELISA) ELISA
Planes R, Pinilla M, Santoni K et al. Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells Molecular cell 2022-05-16 [PMID: 35594856] (ELISA, Human) ELISA
Human
Ruskowski K, Neb H, Talbot SR et al. Persistently Elevated Plasma Levels of RIPK3, MLKL, HMGB1, and RIPK1 in COVID-19 ICU Patients American journal of respiratory cell and molecular biology 2022-04-06 [PMID: 35385375] (ELISA) ELISA
MilAo JFF Utilizing cytotoxic lymphocytes for indirect shock-and-kill strategy in HIV-1 treatment Thesis Jul 13 1905 (ELISA)
ELISA
Atanasova KR, Yilmaz O Inflammasome Activation in Gingival Epithelial Cells Methods in molecular biology (Clifton, N.J.) 2022-02-25 [PMID: 35212963] (ELISA) ELISA
Gonzalez-Chapa JA, PeNa-Martinez VM, VIlchez-Cavazos JF Et al. Systemic and Local Cytokines Profile Determine Severity and Prognosis in Human Septic Arthritis: A Pilot Study Archives of medical research 2021-10-23 [PMID: 34702588] (ELISA) ELISA
Ruskowski K, Neb H, Talbot S Et al. Plasma RIPK3 And HMGB1 Predict Severe COVID-19 Progression In ICU Patients: A Single-Center Cohort Study Research Square 2021-11-19
Vaes RDW, Reynders K, Sprooten J et al. Identification of Potential Prognostic and Predictive Immunological Biomarkers in Patients with Stage I and Stage III Non-Small Cell Lung Cancer (NSCLC): A Prospective Exploratory Study Cancers 2021-12-13 [PMID: 34944879]
Shi H, Gandhi AA, Smith SA Et al. Endothelium-protective, histone-neutralizing properties of the polyanionic agent defibrotide. JCI Insight [PMID: 34264868] (ELISA) ELISA
Vannier AGL, Wardwell B, Fomin V et al. Serum HMGB1 Associates with Liver Disease and Predicts Readmission and Mortality in Patients with Alcohol Use Disorder Alcohol (Fayetteville, N.Y.) 2021-06-09 [PMID: 34118353] (ELISA) ELISA
Sun B, Tang N, Peluso M et al. Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations Cells 2021-02-13 [PMID: 33668514] (ELISA, Human) ELISA
Human
Fan X, Song JW, Wang SY, Cao WJ Changes of Damage Associated Molecular Patterns in COVID-19 Patients Infectious Diseases & Immunity 2021-01-01
Norina Tang, Tatsuo Kido, Jian Shi, Erin McCafferty, Judith M. Ford, Kaitlyn Dal Bon, Lynn Pulliam, Thomas Ichim Blood Markers Show Neural Consequences of LongCOVID-19 Cells 2024-03-08 [PMID: 38534322]
Rubas, NC;Peres, R;Kunihiro, BP;Allan, NP;Phankitnirundorn, K;Wells, RK;McCraken, TA;Lee, RH;Umeda, L;Conching, A;Juarez, R;Maunakea, AK; HMGB1 mediates microbiome-immune axis dysregulation underlying reduced neutralization capacity in obesity-related post-acute sequelae of SARS-CoV-2 Scientific reports 2024-01-03 [PMID: 38172612]
Palacios Y, Ramón-Luing LA, Ruiz A et al. COVID-19 patients with high TNF/IFN-? levels show hallmarks of PANoptosis, an inflammatory cell death Microbes and infection 2023-06-30 [PMID: 37394112] (S-ELISA) S-ELISA
Malty R, Hilbe R, Ahn S et al. Paridiprubart inhibits TLR4-dependant NF-?B activation by multiple pathogens bioRxiv 2023-06-28 (ELISA)
ELISA
Yang, X;Chen, H;Shen, W;Chen, Y;Lin, Z;Zhuo, J;Wang, S;Yang, M;Li, H;He, C;Zhang, X;Hu, Z;Lian, Z;Yang, M;Wang, R;Li, C;Pan, B;Xu, L;Chen, J;Wei, X;Wei, Q;Xie, H;Zheng, S;Lu, D;Xu, X; FGF21 modulates immunometabolic homeostasis via the ALOX15/15-HETE axis in early liver graft injury Nature communications 2024-10-03 [PMID: 39362839]
�zbay Kurt FG, Cicortas BA, Balzasch BM et al. S100A9 and HMGB1 orchestrate MDSC-mediated immunosuppression in melanoma through TLR4 signaling Journal for Immunotherapy of Cancer 2024-01-01 [PMID: 39266214]
Fernando Rodríguez de Fonseca, Francisco Medina-Paz, Mira Sapozhnikov, Isaac Hurtado-Guerrero, Leticia Rubio, Stella Martín-de-las-Heras, Nerea Requena-Ocaña, María Flores-López, María del Mar Fernández-Arjona, Patricia Rivera, Antonia Serrano, Pedro Serrano, Sara C. Zapico, Juan Suárez, Youssef Sari Plasma Concentrations of High Mobility Group Box 1 Proteins and Soluble Receptors for Advanced Glycation End-Products Are Relevant Biomarkers of Cognitive Impairment in Alcohol Use Disorder: A Pilot Study Toxics 2024-02-29 [PMID: 38535924]
Yao Tong, Yinga Wu, Jing Ma, Masataka Ikeda, Tomomi Ide, Courtney T. Griffin, Xi-Qin Ding, Shusheng Wang Comparative mechanistic study of RPE cell death induced by different oxidative stresses Redox Biology 2023-08-06 [PMID: 37566944]
Chang N, DaPrano E, Evans W et al. Neuronal DAMPs exacerbate neurodegeneration via astrocytic RIPK3 signaling bioRxiv 2023-07-24 [PMID: 37546744] (ELISA) ELISA
Khan Z Remote Ischemic Conditioning as an Adjunct Intervention in Traumatic Brain Injury Thesis 2023-01-01 (ELISA)
ELISA
Thais F.C. Fraga-Silva, Ualter G. Cipriano, Marcilio J. Fumagalli, Giseli F. Correa, Carlos A. Fuzo, Douglas dos-Santos, Fabiola L.A.C. Mestriner, Christiane Becari, Andrea Teixeira-Carvalho, Jordana Coelho-dos-Reis, Mayra G. Menegueti, Luiz T.M. Figueiredo, Larissa Dias Cunha, Olindo A. Martins-Filho, Marcelo Dias-Baruffi, Maria Auxiliadora-Martins, Rita C. Tostes, Vania L.D. Bonato Airway epithelial cells and macrophages trigger IL-6-CD95/CD95L axis and mediate initial immunopathology of COVID-19 iScience 2023-10-31 [PMID: 38047070]
Ghosh P, Sasaki K, Pulido Ruiz IA et al. Inflammatory macrophage to hepatocyte signals can be prevented by extracellular vesicle reprogramming Journal of cell science 2023-04-13 [PMID: 37051862] (ELISA, Human) ELISA
Human
Li W Mediators of inflammation in the development of non-alcoholic steatohepatitis and fibrosis Thesis 2023-01-01 (ELISA)
ELISA
Sunil V, Mozhi A, Zhan W et al. In-situ vaccination using dual responsive organelle targeted nanoreactors Biomaterials 2022-11-01 [PMID: 36228516] (ELISA) ELISA
Chen R, Huang Y, Quan J et al. HMGB1 as a potential biomarker and therapeutic target for severe COVID-19 Heliyon 2020-12-01 [PMID: 33313438] (ELISA, Human) ELISA
Human
Wei J, Alfajaro MM, DeWeirdt PC et al. Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection Cell 2020-10-20 [PMID: 33147444] (ELISA, Human) ELISA
Human
Wu K, Feng J, Xiu Y et al. V delta 2 T cell subsets, defined by PD-1 and TIM-3 expression, present varied cytokine responses in acute myeloid leukemia patients Int. Immunopharmacol. 2020-01-16 [PMID: 31955066] (ELISA, Human) ELISA
Human
Cai X, Biswas I, Panicker SR et al. Activated protein C inhibits LPS-mediated acetylation and secretion of HMGB1 in endothelial cells J. Thromb. Haemost. 2019-03-13 [PMID: 30865333] (ELISA, Human) ELISA
Human
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Review for HMGB1/HMG-1 ELISA Kit (NBP2-62766) (1)
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Average Rating: 4
(Based on 1 review)
Reviews using NBP2-62766:
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07/02/2022
Summary
Sample Tested Blood mononuclear cells (PBMCs) Lot FQZ17THT4U
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FAQs for HMGB1/HMG-1 ELISA Kit (NBP2-62766). (Showing 1 - 1 of 1 FAQs).
Can we use the standard material in this HMGB1/HMG-1 ELISA kit past the 6 month expiration date?
Our products do not have a set expiration date, but we guarantee the performance for 6 months if stored properly. If stored properly, the kit will most likely still work past the guarantee date.
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62766_human-hmgb1-hmg-1-elisa-kit-colorimetric-13220241681411.jpg "ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
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![Flow (Intracellular): HMGB1/HMG-1 Antibody [NB100-2322] - An intracellular stain was performed on HeLa with NB100-2322 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](https://images.novusbio.com/images/HMGB1-HMG-1-Antibody-Flow-Intracellular-NB100-2322-img0021.jpg "Flow (Intracellular): HMGB1/HMG-1 Antibody [NB100-2322] - An intracellular stain was performed on HeLa with NB100-2322 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.")