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HLTF Antibody

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Western Blot: HLTF Antibody [NBP1-83256] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: HLTF Antibody [NBP1-83256] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in greeen.
Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Western Blot: HLTF Antibody [NBP1-83256] - Analysis in human cell line HEL.
Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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HLTF Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HLTF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HLTF Protein (NBP1-83256PEP)
Publications
Read Publications using
NBP1-83256 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HLTF Antibody

  • DNA-binding protein/plasminogen activator inhibitor 1 regulator
  • EC 3.6.1
  • EC 3.6.4.-
  • EC 6.3.2.-
  • helicase-like transcription factor
  • HIP116ARING finger protein 80
  • HIP116matrix associated, actin dependent regulator of chromatin
  • RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3
  • subfamily a, member 3
  • Sucrose nonfermenting protein 2-like 3
  • ZBU1

Background

Helicase-like Transcription Factor (HLTF) is a member of the the SWI/SNF family of nuclear enzymes that remove proteins that coil around DNA. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. HLTF proteins aid the stabilization and regulation of the production of proteins in the cell. Inactivation of the HTLF gene may contribute to the transformation of normal colon cells into cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLTF Antibody (NBP1-83256)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, WB.


Filter By Application
IF/IHC
(1)
Immunocytochemistry/ Immunofluorescence
(1)
Immunohistochemistry
(1)
WB
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HLTF
Entrez
Uniprot