![Western Blot: Histone H3.3A Antibody [NBP2-98769] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Western-Blot-NBP2-98769-img0002.jpg "Western Blot: Histone H3.3A Antibody [NBP2-98769] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the human Histone H3.3A. |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H3-3A |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H3.3A Antibody (NBP2-98769)Find related products by research area.
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| Gene Symbol | H3-3A |
![Immunoprecipitation: Histone H3.3A Antibody [NBP2-98769] - Histone H3.3A was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate 2 ul anti-Histone H3.3A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Histone H3.3A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa](http://images.novusbio.com/fullsize/Histone-H3.3A-Antibody-Immunoprecipitation-NBP2-98769-img0001.jpg "Immunoprecipitation: Histone H3.3A Antibody [NBP2-98769] - Histone H3.3A was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate 2 ul anti-Histone H3.3A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Histone H3.3A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")