HIST1H3C Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

HIST1H3C is 136 amino acids long, weighs approximately 15 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, and immunodeficiency. HIST1H3C has also been shown to have interactions with CCDC101, AIRE, HIST1H4A, HIST1H4B, and HIST1H4C in pathways such as the PKA signaling, interferon, amyloids, hemostasis, meiosis, meiotic recombination, and systemic lupus erythematosus pathways.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-07993

• HIST1H3C Lysate